dc.contributor.author | Novitsky, Vladimir | |
dc.contributor.author | Wang, Rui | |
dc.contributor.author | Baca, Jeannie | |
dc.contributor.author | Margolin, Lauren | |
dc.contributor.author | McLane, Mary F. | |
dc.contributor.author | Moyo, Sikhulile | |
dc.contributor.author | Widenfelt, Erik van | |
dc.contributor.author | Makhema, Joseph | |
dc.contributor.author | Essex, M. | |
dc.date.accessioned | 2019-10-15T18:22:17Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Novitsky, Vladimir, Rui Wang, Jeannie Baca, Lauren Margolin, Mary F. McLane, Sikhulile Moyo, Erik van Widenfelt, Joseph Makhema, and M. Essex. 2011. “Evolutionary Gamut of in Vivo Gag Substitutions during Early HIV-1 Subtype C Infection.” Virology 421 (2): 119–28. https://doi.org/10.1016/j.virol.2011.09.020. | |
dc.identifier.issn | 1178-122X | |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:41552036 | * |
dc.description.abstract | Two analyses of HIV-1 subtype C Gag quasispecies were performed in a prospective cohort of 42 acutely and recently infected individuals by SGA on viral RNA/proviral DNA templates. First, in vivo Gag substitutions were assessed in relation to the HIV-1C consensus sequence, which revealed that 29.3% of detected amino acid substitutions can be classified as reversions to subtype consensus, 61.3% as forward substitutions from subtype consensus, and 9.3% as polymorphisms not associated with the subtype consensus sequence. Second, the proportion, dynamics, and relationships within individual pools of viral quasispecies were analyzed. Among reverse substitutions, 16.1% were minor, 11.0% transient, 13.6% dominant, and 59.2% fixed. In contrast, 31.6% of forward substitutions were minor, 59.3% transient, 3.8% dominant, and 5.3% fixed. The distinct patterns in the spectrum and dynamics of reverse and forward Gag substitutions suggest that these differences should be considered in HIV-1 evolutionary studies and analyses of viral mutational pathways. | |
dc.language.iso | en_US | |
dc.publisher | SAGE Publications | |
dash.license | LAA | |
dc.title | Evolutionary Gamut of in vivo Gag Substitutions during Early HIV-1 Subtype C Infection | |
dc.type | Journal Article | |
dc.description.version | Accepted Manuscript | |
dc.relation.journal | Virology - Research and Treatment | |
dash.depositing.author | Wang, Rui::bf1a234ca95ae1f0959d1c158d4f8aa4::600 | |
dc.date.available | 2019-10-15T18:22:17Z | |
dash.workflow.comments | 1Science Serial ID 115885 | |
dc.identifier.doi | 10.1016/j.virol.2011.09.020 | |
dash.source.volume | 421;2 | |
dash.source.page | 119 | |