Mycobacterial Esx-3 is required for mycobactin-mediated iron acquisition
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Author
Siegrist, M. Sloan
Unnikrishnan, Meera
McConnell, Matthew J.
Borowsky, Mark
Cheng, Tan-Yun
Siddiqi, Noman
Fortune, Sarah M.
Moody, D. Branch
Rubin, Eric J.
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https://doi.org/10.1073/pnas.0900589106Metadata
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Siegrist, M. S., M. Unnikrishnan, M. J. McConnell, M. Borowsky, T.-Y. Cheng, N. Siddiqi, S. M. Fortune, D. B. Moody, and E. J. Rubin. 2009. “Mycobacterial Esx-3 Is Required for Mycobactin-Mediated Iron Acquisition.” Proceedings of the National Academy of Sciences 106 (44): 18792–97. https://doi.org/10.1073/pnas.0900589106.Abstract
The Esx secretion pathway is conserved across Gram-positive bacteria. Esx-1, the best-characterized system, is required for virulence of Mycobacterium tuberculosis, although its precise function during infection remains unclear. Esx-3, a paralogous system present in all mycobacterial species, is required for growth in vitro. Here, we demonstrate that mycobacteria lacking Esx-3 are defective in acquiring iron. To compete for the limited iron available in the host and the environment, these organisms use mycobactin, high-affinity iron-binding molecules. In the absence of Esx-3, mycobacteria synthesize mycobactin but are unable to use the bound iron and are impaired severely for growth during macrophage infection. Mycobacteria thus require a specialized secretion system for acquiring iron from siderophores.Terms of Use
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