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dc.contributor.authorJavid, Babak
dc.contributor.authorSorrentino, Flavia
dc.contributor.authorToosky, Melody
dc.contributor.authorZheng, Wen
dc.contributor.authorPinkham, Jessica T.
dc.contributor.authorJain, Nina
dc.contributor.authorPan, Miaomiao
dc.contributor.authorDeighan, Padraig
dc.contributor.authorRubin, Eric J.
dc.date.accessioned2019-10-15T18:22:29Z
dc.date.issued2014
dc.identifier.citationJavid, B., F. Sorrentino, M. Toosky, W. Zheng, J. T. Pinkham, N. Jain, M. Pan, P. Deighan, and E. J. Rubin. 2014. “Mycobacterial Mistranslation Is Necessary and Sufficient for Rifampicin Phenotypic Resistance.” Proceedings of the National Academy of Sciences 111 (3): 1132–37. https://doi.org/10.1073/pnas.1317580111.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41552054*
dc.description.abstractErrors are inherent in all biological systems. Errors in protein translation are particularly frequent giving rise to a collection of protein quasi-species, the diversity of which will vary according to the error rate. As mistranslation rates rise, these new proteins could produce new phenotypes, although none have been identified to date. Here, we find that mycobacteria substitute glutamate for glutamine and aspartate for asparagine at high rates under specific growth conditions. Increasing the substitution rate results in remarkable phenotypic resistance to rifampicin, whereas decreasing mistranslation produces increased susceptibility to the antibiotic. These phenotypic changes are reflected in differential susceptibility of RNA polymerase to the drug. We propose that altering translational fidelity represents a unique form of environmental adaptation.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleMycobacterial mistranslation is necessary and sufficient for rifampicin phenotypic resistance
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorRubin, Eric J.::2ccf3691d766f02c048797e00c06cc44::600
dc.date.available2019-10-15T18:22:29Z
dash.workflow.comments1Science Serial ID 91186
dc.identifier.doi10.1073/pnas.1317580111
dash.source.volume111;3
dash.source.page1132


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