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dc.contributor.authorWerley, Christopher
dc.contributor.authorChien, Miao-ping
dc.contributor.authorCohen, Adam
dc.date.accessioned2019-11-04T22:27:13Z
dc.date.issued2017-11-29
dc.identifier.citationWerley, Christopher A., Miao-Ping Chien, and Adam E. Cohen. 2017. Ultrawidefield Microscope for High-speed Fluorescence Imaging and Targeted Optogenetic Stimulation. Biomedical Optics Express 8, no. 12: 5794-5813.en_US
dc.identifier.issn2156-7085en_US
dc.identifier.issn2156-7085en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41718758*
dc.description.abstractThe rapid increase in the number and quality of fluorescent reporters and optogenetic actuators has yielded a powerful set of tools for recording and controlling cellular state and function. To achieve the full benefit of these tools requires improved optical systems with high light collection efficiency, high spatial and temporal resolution, and patterned optical stimulation, in a wide field of view (FOV). Here we describe our 'Firefly' microscope, which achieves these goals in a Ø6 mm FOV. The Firefly optical system is optimized for simultaneous photostimulation and fluorescence imaging in cultured cells. All but one of the optical elements are commercially available, yet the microscope achieves 10-fold higher light collection efficiency at its design magnification than the comparable commercially available microscope using the same objective. The Firefly microscope enables all-optical electrophysiology ('Optopatch') in cultured neurons with a throughput and information content unmatched by other neuronal phenotyping systems. This capability opens possibilities in disease modeling and phenotypic drug screening. We also demonstrate applications of the system to voltage and calcium recordings in human induced pluripotent stem cell derived cardiomyocytes.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dc.publisherThe Optical Societyen_US
dash.licenseOAP
dc.subjectBiotechnologyen_US
dc.subjectAtomic and Molecular Physics, and Opticsen_US
dc.titleUltrawidefield Microscope for High-Speed Fluorescence Imaging and Targeted Optogenetic Stimulationen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalBiomedical Optics Expressen_US
dash.depositing.authorCohen, Adam
dc.date.available2019-11-04T22:27:13Z
dash.workflow.commentsFAR2017en_US
dash.funder.nameHoward Hughes Medical Instituteen_US
dash.funder.nameNIHen_US
dash.funder.nameONRen_US
dash.funder.nameGordon and Betty Moore Foundationen_US
dash.funder.award1-R01 EB012498en_US
dash.funder.awardN000141110-549en_US
dc.identifier.doi10.1364/boe.8.005794
dc.source.journalBiomed. Opt. Express
dash.source.volume8;12
dash.source.page5794
dash.contributor.affiliatedChien, Miao-ping
dash.contributor.affiliatedCohen, Adam


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