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dc.contributor.authorJalili-Firoozinezhad, Sasan
dc.contributor.authorGazzaniga, Francesca
dc.contributor.authorCalamari, Elizabeth
dc.contributor.authorCamacho, Diogo
dc.contributor.authorFadel, Cicely
dc.contributor.authorBein, Amir
dc.contributor.authorSwenor, Ben
dc.contributor.authorNestor, Bret
dc.contributor.authorCronce, Michael
dc.contributor.authorLevy, Oren
dc.contributor.authorGregory, Katherine
dc.contributor.authorBreault, David
dc.contributor.authorCabral, Joaquim
dc.contributor.authorNovak, Richard
dc.contributor.authorKasper, Dennis
dc.contributor.authorTovaglieri, Alessio
dc.contributor.authorIngber, Donald
dc.date.accessioned2019-11-06T22:16:30Z
dc.date.issued2019-07
dc.identifier.citationJalili-Firoozinezhad, Sasan, Francesca S. Gazzaniga, Elizabeth L. Calamari, Diogo M. Camacho, Cicely W. Fadel, Amir Bein, Ben Swenor, Bret Nestor, Michael J. Cronce, Alessio Tovaglieri, Oren Levy, Katherine E. Gregory, David T. Breault, Joaquim M. S. Cabral, Dennis L. Kasper, Richard Novak, and Donald E. Ingber. 2019. A Complex Human Gut Microbiome Cultured in an Anaerobic Intestine-on-a-chip. Nature Biomedical Engineering 3, no. 7: 520-31.en_US
dc.identifier.issn2157-846Xen_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41759414*
dc.description.abstractThe diverse bacterial populations that comprise the commensal microbiome of the human intestine play a central role in health and disease. A method that sustains complex microbial communities in direct contact with living human intestinal cells and their overlying mucus layer in vitro would thus enable the investigation of host–microbiome interactions. Here, we show the extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota, using a microfluidic intestine-on-a-chip that permits the control and real-time assessment of physiologically relevant oxygen gradients. When compared to aerobic coculture conditions, the establishment of a transluminal hypoxia gradient in the chip increased intestinal barrier function and sustained a physiologically relevant level of microbial diversity, consisting of over 200 unique operational taxonomic units from 11 different genera and an abundance of obligate anaerobic bacteria, with ratios of Firmicutes and Bacteroidetes similar to those observed in human faeces. The intestine-on-a-chip may serve as a discovery tool for the development of microbiome-related therapeutics, probiotics and nutraceuticals.en_US
dc.language.isoen_USen_US
dc.publisher‎Nature Researchen_US
dc.relationNature: Biomedical Engineeringen_US
dc.relation.isversionofdoi: 10.1038/s41551-019-0397-0en_US
dc.relation.hasversionhttps://www.ncbi.nlm.nih.gov/pubmed/31086325en_US
dash.licenseMETA_ONLY
dc.titleA Complex Human-Gut Microbiome Cultured in an Anaerobic Intestine-on-a-Chipen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNature Biomedical Engineeringen_US
dash.depositing.authorKasper, Dennis
dash.waiver2019-10-08
dc.date.available2019-11-06T22:16:30Z
dash.affiliation.otherHarvard Medical Schoolen_US
dash.workflow.commentsFAR2017en_US
dc.identifier.doi10.1038/s41551-019-0397-0*
dash.waiver.reasonRequested by publisher.en_US
dash.contributor.affiliatedCabral, Joaquim
dash.contributor.affiliatedGazzaniga, Francesca
dash.contributor.affiliatedCalamari, Elizabeth
dash.contributor.affiliatedCamacho, Diogo
dash.contributor.affiliatedFadel, Cicely
dash.contributor.affiliatedSwenor, Ben
dash.contributor.affiliatedGregory, Katherine
dash.contributor.affiliatedNovak, Richard
dash.contributor.affiliatedTovaglieri, Alessio
dash.contributor.affiliatedBein, Amir
dash.contributor.affiliatedIngber, Donald
dash.contributor.affiliatedBreault, David
dash.contributor.affiliatedKasper, Dennis


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