Show simple item record

dc.contributor.advisorAndermann, Mark
dc.contributor.authorWelsh, Christina Aina Maria
dc.date.accessioned2019-12-12T08:00:38Z
dc.date.created2019-05
dc.date.issued2019-04-17
dc.date.submitted2019
dc.identifier.citationWelsh, Christina Aina Maria. 2019. Mechanisms of Microglia-Synapse Interactions in Visual System Development and Plasticity. Doctoral dissertation, Harvard University, Graduate School of Arts & Sciences.
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:42029458*
dc.description.abstractMicroglia and immune genes are required for the proper development and plasticity of the mouse visual system. Microglia, the resident macrophages of the brain parenchyma, promote developmental synapse elimination by engulfing synapses, and many genes originally identified through their contributions to immunity are important for visual system development and plasticity. The mechanisms underlying microglia and immune gene interactions with developing synapses are, however, poorly understood. We therefore assessed the contributions of three different immune signaling pathways to synaptic development in the mouse visual system. We first tested in vitro and in vivo strategies for studying microglial synapse engulfment. We developed an in vitro engulfment assay, and used it to elucidate signaling between the neuronal ligand CD47 and the microglial receptor SIRPα. We next investigated whether the pro- engulfment classical complement cascade is required for ocular dominance plasticity, by examining aspects of binocular visual cortex development and plasticity in mice lacking C1q. Surprisingly, we found that visual cortex is mostly normal in the absence of C1q, with only mild, experience-dependent synaptic phenotypes detectable in these animals. Finally, we studied whether CD200, an immune-suppressant protein expressed in many tissues including the brain, is required for proper retinogeniculate refinement. In contrast to previous results, we were not able to detect a refinement phenotype in mice lacking CD200. Together, these results illustrate the complexity of the molecular mechanisms underlying immune system contributions to visual system development and plasticity.
dc.description.sponsorshipMedical Sciences
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjectmicroglia
dc.subjectvisual system
dc.subjectneuro-immunology
dc.subjectdevelopment
dc.titleMechanisms of Microglia-Synapse Interactions in Visual System Development and Plasticity
dc.typeThesis or Dissertation
dash.depositing.authorWelsh, Christina Aina Maria
dc.date.available2019-12-12T08:00:38Z
thesis.degree.date2019
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.levelDoctoral
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
thesis.degree.nameDoctor of Philosophy
dc.contributor.committeeMemberGoodrich, Lisa
dc.contributor.committeeMemberHeiman, Maxwell
dc.contributor.committeeMemberGreer, Paul
dc.type.materialtext
thesis.degree.departmentMedical Sciences
thesis.degree.departmentMedical Sciences
dash.identifier.vireo
dc.identifier.orcid0000-0001-9802-725X
dash.author.emailchristinaam.welsh@gmail.com


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record