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dc.contributor.advisorLosick, Richard
dc.contributor.advisorDesai, Michael
dc.contributor.advisorWeiss, Ron
dc.contributor.authorKim, Suhyun
dc.date.accessioned2019-12-12T09:07:01Z
dc.date.created2019-05
dc.date.issued2019-05-16
dc.date.submitted2019
dc.identifier.citationKim, Suhyun. 2019. Quorum sensing can be repurposed to promote information transfer between bacteria in the mammalian gut. Doctoral dissertation, Harvard University, Graduate School of Arts & Sciences.
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:42029725*
dc.description.abstractThe gut microbiome is intricately involved with establishing and maintaining the health of the host. Engineering of gut microbes aims to add new functions and expand the scope of control over the gut microbiome. To create systems that can perform increasingly complex tasks in the gut, it is necessary to first harness the ability of the bacteria to communicate in the gut environment. Interestingly, acyl-homoserine lactone (acyl-HSL)-mediated Gram-negative bacterial quorum sensing, a widely-used mode of inter-cellular signaling system in nature, has not been identified in normal healthy mammalian gut. It remains unknown whether the gut bacteria that do not natively use quorum sensing can be engineered to successfully signal to other bacteria using acyl-HSLs in the gut despite the inherent challenges imposed by this complex environment. Here, I repurposed quorum sensing to create an information transfer system between native gut Escherichia coli and attenuated Salmonella enterica serovar Typhimurium. Specifically, I functionalized one species with inducible signal production and the other with signal detection and recording using genomically integrated circuits. The information transfer system demonstrated successful intra-and inter-species signaling in the murine gut. This study provides a basis for further understanding of inter-bacterial interactions in an otherwise hard-to-study environment as well as a basis for further investigation of the potential of acyl-HSLs as inter-cellular signaling molecules of engineered gut consortia.
dc.description.sponsorshipBiology, Molecular and Cellular
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjectMicrobiome engineering
dc.subjectquorum sensing
dc.titleQuorum sensing can be repurposed to promote information transfer between bacteria in the mammalian gut
dc.typeThesis or Dissertation
dash.depositing.authorKim, Suhyun
dc.date.available2019-12-12T09:07:01Z
thesis.degree.date2019
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.levelDoctoral
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
thesis.degree.nameDoctor of Philosophy
dc.type.materialtext
thesis.degree.departmentBiology, Molecular and Cellular
thesis.degree.departmentBiology, Molecular and Cellular
dash.identifier.vireo
dc.identifier.orcid0000-0002-4211-1046
dash.author.emailisilierinde@gmail.com


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