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dc.contributor.authorKallgren, Scott Peter
dc.date.accessioned2019-12-17T09:22:45Z
dash.embargo.terms2018-05-01
dc.date.created2016-05
dc.date.issued2016-07-27
dc.date.submitted2016
dc.identifier.citationKallgren, Scott Peter. 2016. Spt5 Regulates Sense and Antisense Transcription Genome-Wide. Master's thesis, Harvard Medical School.
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:42061443*
dc.description.abstractSpt5 is the only transcription elongation factor conserved in all three domains of life, but its molecular mechanisms are not yet thoroughly studied genomically. From an inducible depletion strain, we sequenced nascent transcripts (NET-seq), mature mRNA (RNA-seq), and RNA polymerase II-associated chromatin (ChIP-seq) to elucidate general effects of Spt5 on transcription. These show an increase in 5’ CDS antisense transcription by RNA-seq and NET-seq and a general accumulation of RNA Pol II at the 5’ ends of genes by exogenous spike-in-normalized ChIP-seq. That similar antisense transcripts appear in RNA-seq and NET-seq indicates that novel antisense transcripts are resulting from new transcription rather than aberrant decay. These results provide insight into how Spt5 functions to facilitate RNA Pol II elongation in diverse organisms.
dc.description.sponsorshipMaster of Medical Sciences in Biomedical Informatics
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjecttranscription
dc.subjectelongation
dc.subjectantisense
dc.subjectNET-seq
dc.titleSpt5 Regulates Sense and Antisense Transcription Genome-Wide
dc.typeThesis or Dissertation
dash.depositing.authorKallgren, Scott Peter
dash.embargo.until2018-05-01
dc.date.available2019-12-17T09:22:45Z
thesis.degree.date2016
thesis.degree.grantorHarvard Medical School
thesis.degree.grantorHarvard Medical School
thesis.degree.levelMasters
thesis.degree.levelMasters
thesis.degree.nameMaster of Medical Sciences
thesis.degree.nameMaster of Medical Sciences
dc.contributor.committeeMemberPark, Peter J.
dc.contributor.committeeMemberWinston, Fred M.
dc.contributor.committeeMemberKharchenko, Peter V.
dc.type.materialtext
dash.identifier.vireo
dc.identifier.orcid0000-0002-6318-3268
dash.author.emailskallgren@gmail.com


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