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dc.contributor.authorFisher, Jonathan
dc.date.accessioned2019-12-18T08:41:16Z
dc.date.created2019-05
dc.date.issued2019-06-28
dc.date.submitted2019
dc.identifier.citationFisher, Jonathan. 2019. Stem Cell Hierarichies within Tumor Genetic Subclones. Doctoral dissertation, Harvard Medical School.
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:42063324*
dc.description.abstractIntratumoral heterogeneity has been appreciated at the genetic and transcriptional levels, yet it remains unclear how they relate to one another. My thesis project applies a novel qPCR technique to combine single-cell RNA-seq with same-cell cDNA genotyping at single-nucleotide resolution. Using whole-exome sequencing in a human oligodendroglioma tumor, we identified a mutation in CIC that is present in only a subset of cancer cells. A previous analysis of single-cell RNA-seq in this tumor showed a substantial minority of cancer cells that display a stemness phenotype distinct from differentiated lineage phenotypes previously known in low-grade glioma. Results of parallel qPCR genotyping demonstrate that both CIC-wild-type and CIC-mutant cells are found across the spectrum of stem-differentiated phenotypes. This suggests that the stemness phenotype exists independently of mutation status and that it is instead more likely epigenetically driven.
dc.description.sponsorshipHealth Sciences and Technology
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjectsingle-cell RNA-seq
dc.subjectoligodendroglioma
dc.subjectglioma
dc.subjecttumor heterogeneity
dc.subjectcancer stem cells
dc.titleStem Cell Hierarichies within Tumor Genetic Subclones
dc.typeThesis or Dissertation
dash.depositing.authorFisher, Jonathan
dc.date.available2019-12-18T08:41:16Z
thesis.degree.date2019
thesis.degree.grantorHarvard Medical School
thesis.degree.grantorHarvard Medical School
thesis.degree.levelDoctoral
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Medicine
thesis.degree.nameDoctor of Medicine
dc.type.materialtext
dash.identifier.vireo
dash.author.emailfisher.jonathanm@gmail.com


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