Show simple item record

dc.contributor.authorWang, Kathy K.
dc.date.accessioned2019-12-19T08:34:45Z
dash.embargo.terms2020-05-01
dc.date.created2019-05
dc.date.issued2019-05-06
dc.date.submitted2019
dc.identifier.citationWang, Kathy K. 2019. Interleukin-33-Producing Stromal Cells Mediate Crosstalk Between Neuronal and Regulatory T Cells in Murine Skeletal Muscle. Doctoral dissertation, Harvard Medical School.
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:42069353*
dc.description.abstractA distinct population of regulatory T cells (Tregs) that accumulates in injured skeletal muscle helps control inflammation and enhances muscle regeneration. Muscle-localized Tregs express high levels of ST2, which encodes the receptor for the alarmin IL-33. The signaling of IL-33 through ST2 has been shown to promote Treg proliferation and support muscle repair. Here, we found that the primary IL-33 producers in mouse skeletal muscle are a subset of mesenchymal stromal cells (MSCs) expressing the markers Sca-1 and PDGFRα. We observed that IL-33-producing MSCs are often closely associated with peripheral nerve bundles as well as small-diameter sensory fibers. Population- and single-cell RNA-seq analysis revealed that the receptor for a pain-related neuropeptide, calcitonin gene-related peptide (CGRP), is expressed in IL-33+ cells. Furthermore, in vivo pharmacological perturbations suggested a role for CGRP in modulating IL-33 production in mouse skeletal muscle MSCs, thereby revealing the presence of a sensory neuron-immune interaction within the skeletal muscle environment that is mediated by IL33+ stromal cells.
dc.description.sponsorshipHonors in a Special Field
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjectIL-33
dc.subjectTregs
dc.subjectsensory neurons
dc.subjecttissue repair
dc.subjectskeletal muscle
dc.titleInterleukin-33-Producing Stromal Cells Mediate Crosstalk Between Neuronal and Regulatory T Cells in Murine Skeletal Muscle
dc.typeThesis or Dissertation
dash.depositing.authorWang, Kathy K.
dash.embargo.until2020-05-01
dc.date.available2019-12-19T08:34:45Z
thesis.degree.date2019
thesis.degree.grantorHarvard Medical School
thesis.degree.grantorHarvard Medical School
thesis.degree.levelDoctoral
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Medicine
thesis.degree.nameDoctor of Medicine
dc.type.materialtext
dash.identifier.vireo
dash.author.emailkaixi213@gmail.com


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record