A Novel Injectable Gel Incorporating Human Platelet Rich Plasma (PRP) Lysate for Periodontal Regeneration: In Vitro Studies
VALVERDE, LIVIA R. P.
MetadataShow full item record
CitationVALVERDE, LIVIA R. P. 2019. A Novel Injectable Gel Incorporating Human Platelet Rich Plasma (PRP) Lysate for Periodontal Regeneration: In Vitro Studies. Doctoral dissertation, Harvard School of Dental Medicine.
AbstractBackground: Platelet rich plasma (PRP) has been utilized as an adjunct to various periodontal procedures with the goal of potentializing the regenerative outcome of surgical therapy. The biopolymer gelatin hydroxylphenylpropionic acid (Gtn-HPA) has recently been developed and proposed to be used as an injectable scaffold for tissue engineering. The aim of the present study was to characterize the influence of the Gtn-HPA hydrogel incorporating PRP on osteoprogenitor cells behavior and growth factor release in vitro.
Methods: Platelet rich plasma was obtained from 6 human donors followed by platelet counting. The mRNA levels of osteoblast differentiation markers including OCN and COL1A1 plus the growth factors receptors FGFr, EGFr and PDGFr were analyzed in 2D cultures, hydrogel only, 10% PRP, 20% PRP, 10% FBS and 20% FBS in a 2% Gtn-HPA hydrogel (n=3) and seeded with osteoprogenitor cells. Furthermore, protein quantification for PDGF, TGF-B, EGF, FGF and IL-1B (n=5) were analyzed in the PRP lysate samples.
Results: Platelet concentration increased between 5 to 9-fold in PRP compared to whole blood counts. Protein quantification was consistently greater for TGFB, PDGF and EGF. Gene expression analysis showed a tendency of upregulation of the osteoblast differentiation markers and growth factor receptor genes in the PRP groups, however a great variability between the donors was noticed.
Conclusion: Within the limitations of this study, the use of human PRP in a Gtn-HPA hydrogel reveals this biopolymer as a promising and suitable carrier/scaffold with optimal growth factor release and enhanced gene expression favoring tissue regeneration.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:42080534