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dc.contributor.authorOstrov, Nili
dc.contributor.authorLee, Henry
dc.contributor.authorWong, Brandon
dc.contributor.authorGold, Michaela
dc.contributor.authorKhalil, Ahmad
dc.contributor.authorChurch, George
dc.date.accessioned2019-12-27T19:33:51Z
dc.date.issued2019-04-08
dc.identifier.citationLee, Henry H., Nili Ostrov, Brandon G. Wong, Michaela A. Gold, Ahmad S. Khalil, and George M. Church.2019. Functional Genomics of the Rapidly Replicating Bacterium Vibrio Natriegens by CRISPRi. Nature Microbiology 4, no. 7: 1105-113.en_US
dc.identifier.issn2058-5276en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:42083015*
dc.description.abstractThe fast growing Gram-negative bacterium Vibrio natriegens is an attractive microbial system for molecular biology and biotechnology due to its remarkable short generation time1,2 and metabolic prowess3,4. However, efforts to uncover and utilize the mechanisms underlying its rapid growth are hampered by the scarcity of functional genomic data. Here, we develop a pooled genome-wide CRISPR interference (CRISPRi) screen to identify a minimal set of genes required for rapid wild-type growth. Targeting 4,565 (99.7%) of predicted protein-coding genes, our screen uncovered core genes composed of putative essentials and growth-supporting genes which are enriched for respiratory pathways. We found 96% of core genes to be located on the larger chromosome 1, with growth-neutral duplicates of core gene located primarily on chromosome 2. Our screen also refines metabolic pathway annotations by distinguishing functional biosynthetic enzymes from those predicted based on comparative genomics. Taken together, this work provides a broadly applicable platform for high-throughput functional genomics to accelerate biological studies and engineering of V. natriegens.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relationNature Microbiologyen_US
dash.licenseLAA
dc.subjectImmunologyen_US
dc.subjectMicrobiology (medical)en_US
dc.subjectApplied Microbiology and Biotechnologyen_US
dc.subjectGeneticsen_US
dc.subjectCell Biologyen_US
dc.subjectMicrobiologyen_US
dc.titleFunctional Genomics of the Rapidly Replicating Bacterium Vibrio Natriegens by CRISPRien_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNature Microbiologyen_US
dash.depositing.authorChurch, George
dash.waiver2019-02-22
dc.date.available2019-12-27T19:33:51Z
dash.affiliation.otherHarvard Business Schoolen_US
dc.identifier.doi10.1038/s41564-019-0423-8
dc.source.journalNat Microbiol
dash.waiver.reasonFrom Nature Microbiology editor: We have noted that one or more authors of the manuscript are from an institution that has an open access policy in place – which is incompatible with the business model for this journal. As these authors are subject to their institutions’ respective open access policies and we will need you to provide us with a waiver from the institution of every affected author on the manuscript.( Harvard)en_US
dash.source.volume4;7
dash.source.page1105-1113
dash.contributor.affiliatedOstrov, Nili
dash.contributor.affiliatedKhalil, Ahmad
dash.contributor.affiliatedLee, Henry
dash.contributor.affiliatedChurch, George


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