MicroRNA miR-125a Controls Hematopoietic Stem Cell Number

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MicroRNA miR-125a Controls Hematopoietic Stem Cell Number

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Title: MicroRNA miR-125a Controls Hematopoietic Stem Cell Number
Author: Guo, Shangqin; Lu, Jun; Schlanger, Rita; Zhang, Hao; Wang, Judy Y.; Fox, Michelle Caroline; Purton, Louise E.; Fleming, Heather H.; Cobb, Bradley; Merkenschlager, Matthias; Golub, Todd R.; Scadden, David Thomas

Note: Order does not necessarily reflect citation order of authors.

Citation: Guo, Shangqin, Jun Lu, Rita Schlanger, Hao Zhang, Judy Y. Wang, Michelle C. Fox, Louise E. Purton, et al. 2010. MicroRNA miR-125a controls hematopoietic stem cell number. Proceedings of the National Academy of Sciences 107(32): 14229-14234.
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Abstract: MicroRNAs influence hematopoietic differentiation, but little is known about their effects on the stem cell state. Here, we report that the microRNA processing enzyme Dicer is essential for stem cell persistence in vivo and a specific microRNA, miR-125a, controls the size of the stem cell population by regulating hematopoietic stem/progenitor cell (HSPC) apoptosis. Conditional deletion of Dicer revealed an absolute dependence for the multipotent HSPC population in a cell-autonomous manner, with increased HSPC apoptosis in mutant animals. An evolutionarily conserved microRNA cluster containing miR-99b, let-7e, and miR-125a was preferentially expressed in long-term hematopoietic stem cells. MicroRNA miR-125a alone was capable of increasing the number of hematopoietic stem cells in vivo by more than 8-fold. This result was accomplished through a differentiation stage-specific reduction of apoptosis in immature hematopoietic progenitors, possibly through targeting multiple proapoptotic genes. Bak1 was directly down-regulated by miR-125a and expression of a 3′UTR-less Bak1 blocked miR-125a-induced hematopoietic expansion in vivo. These data demonstrate cell-state-specific regulation by microRNA and identify a unique microRNA functioning to regulate the stem cell pool size.
Published Version: doi:10.1073/pnas.0913574107
Other Sources: http://www.pnas.org/content/early/2010/07/06/0913574107.full.pdf+html
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4341695
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