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dc.contributor.authorKingsley, Evan Prentice
dc.contributor.authorManceau, Marie C.
dc.contributor.authorWiley, Christopher D.
dc.contributor.authorHoekstra, Hopi E.
dc.date.accessioned2010-09-30T17:17:02Z
dc.date.issued2009
dc.identifier.citationKingsley, Evan P., Marie Manceau, Christopher D. Wiley, and Hopi E. Hoekstra. 2009. Melanism in Is Caused by Independent Mutations in Agouti. PLoS ONE 4(7): e6435.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4454675
dc.description.abstractIdentifying the molecular basis of phenotypes that have evolved independently can provide insight into the ways genetic and developmental constraints influence the maintenance of phenotypic diversity. Melanic (darkly pigmented) phenotypes in mammals provide a potent system in which to study the genetic basis of naturally occurring mutant phenotypes because melanism occurs in many mammals, and the mammalian pigmentation pathway is well understood. Spontaneous alleles of a few key pigmentation loci are known to cause melanism in domestic or laboratory populations of mammals, but in natural populations, mutations at one gene, the melanocortin-1 receptor (Mc1r), have been implicated in the vast majority of cases, possibly due to its minimal pleiotropic effects. To investigate whether mutations in this or other genes cause melanism in the wild, we investigated the genetic basis of melanism in the rodent genus Peromyscus, in which melanic mice have been reported in several populations. We focused on two genes known to cause melanism in other taxa, Mc1r and its antagonist, the agouti signaling protein (Agouti). While variation in the Mc1r coding region does not correlate with melanism in any population, in a New Hampshire population, we find that a 125-kb deletion, which includes the upstream regulatory region and exons 1 and 2 of Agouti, results in a loss of Agouti expression and is perfectly associated with melanic color. In a second population from Alaska, we find that a premature stop codon in exon 3 of Agouti is associated with a similar melanic phenotype. These results show that melanism has evolved independently in these populations through mutations in the same gene, and suggest that melanism produced by mutations in genes other than Mc1r may be more common than previously thought.en_US
dc.description.sponsorshipOrganismic and Evolutionary Biologyen_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0006435en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713407/pdf/en_US
dash.licenseOAP
dc.subjectevolutionary biologyen_US
dc.subjectanimal geneticsen_US
dc.subjectdevelopmental evolutionen_US
dc.subjectevolutionary ecologyen_US
dc.subjectgenetics and genomicsen_US
dc.titleMelanism in Peromyscus Is Caused by Independent Mutations in Agoutien_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorHoekstra, Hopi E.
dc.date.available2010-09-30T17:17:02Z
dc.identifier.doi10.1371/journal.pone.0006435*
dash.contributor.affiliatedKingsley, Evan
dash.contributor.affiliatedManceau, Marie
dash.contributor.affiliatedHoekstra, Hopi


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