Browsing Harvard Medical School by Author "Qadri, Firdausi"
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Analysis of Salmonella enterica Serotype Paratyphi A Gene Expression in the Blood of Bacteremic Patients in Bangladesh
Sheikh, Alaullah; Bhuiyan, Md. Saruar; Khanam, Farhana; Bukka, Archana; Kalsy, Anuj; Porwollik, Steffen; Cravioto, Alejandro; Logvinenko, Tanya; McClelland, Michael; Graham, James E.; Qadri, Firdausi; Charles, Richelle Candice; Rollins, Sean McKenzie; Harris, Jason B.; Brooks, W. Abdullah; Larocque, Regina Celes; Hohmann, Elizabeth Louise; Calderwood, Stephen Beaven; Ryan, Edward Thomas (Public Library of Science, 2010)Background: Salmonella enterica serotype Paratyphi A is a human-restricted cause of paratyphoid fever, accounting for up to a fifth of all cases of enteric fever in Asia. Methodology/Principal Findings: In this work, we ... -
Antibody Avidity in Humoral Immune Responses in Bangladeshi Children and Adults following Administration of an Oral Killed Cholera Vaccine
Alam, Mohammad Murshid; Leung, Daniel T.; Akhtar, Marjahan; Nazim, Mohammad; Akter, Sarmin; Uddin, Taher; Khanam, Farhana; Mahbuba, Deena Al; Ahmad, Shaikh Meshbahuddin; Bhuiyan, Taufiqur Rahman; Calderwood, Stephen B.; Ryan, Edward T.; Qadri, Firdausi (American Society for Microbiology, 2013)Antibody avidity for antigens following disease or vaccination increases with affinity maturation and somatic hypermutation. In this study, we followed children and adults in Bangladesh for 1 year following oral cholera ... -
Antibody Secreting Cell Responses following Vaccination with Bivalent Oral Cholera Vaccine among Haitian Adults
Matias, Wilfredo R.; Falkard, Brie; Charles, Richelle C.; Mayo-Smith, Leslie M.; Teng, Jessica E.; Xu, Peng; Kováč, Pavol; Ryan, Edward T.; Qadri, Firdausi; Franke, Molly F.; Ivers, Louise C.; Harris, Jason B. (Public Library of Science, 2016)Background: The bivalent whole-cell (BivWC) oral cholera vaccine (Shanchol) is effective in preventing cholera. However, evaluations of immune responses following vaccination with BivWC have been limited. To determine ... -
Antibody-Secreting Cell Responses after Vibrio cholerae O1 Infection and Oral Cholera Vaccination in Adults in Bangladesh
Rahman, Atiqur; Rashu, Rasheduzzaman; Bhuiyan, Taufiqur Rahman; Chowdhury, Fahima; Khan, Ashraful Islam; Islam, Kamrul; LaRocque, Regina C.; Ryan, Edward T.; Wrammert, Jens; Calderwood, Stephen B.; Qadri, Firdausi; Harris, Jason B. (American Society for Microbiology, 2013)Infection with Vibrio cholerae and oral cholera vaccines (OCVs) induce transient circulating plasmablast responses that peak within approximately 7 days after infection or vaccination. We previously demonstrated that ... -
Antigen-Specific Immunoglobulin a Antibodies Secreted from Circulating B Cells Are an Effective Marker for Recent Local Immune Responses in Patients with Cholera: Comparison to Antibody-Secreting Cell Responses and Other Immunological Markers
Qadri, Firdausi; Ryan, Edward T.; Faruque, A. G.; Ahmed, Firoz; Khan, Ashraful Islam; Islam, M. Monirul; Akramuzzaman, Syed M.; Sack, David. A.; Calderwood, Stephen B. (American Society for Microbiology, 2003)Gut-derived lymphocytes transiently migrate through the peripheral circulation before homing back to mucosal sites and can be detected using an ELISPOT-based antibody secreting cell (ASC) assay. Alternatively, transiently ... -
Antigen-Specific Memory B-Cell Responses in Bangladeshi Adults after One- or Two-Dose Oral Killed Cholera Vaccination and Comparison with Responses in Patients with Naturally Acquired Cholera
Alam, Mohammad Murshid; Riyadh, M. Asrafuzzaman; Fatema, Kaniz; Rahman, Mohammad Arif; Akhtar, Nayeema; Ahmed, Tanvir; Chowdhury, Mohiul Islam; Chowdhury, Fahima; Calderwood, Stephen B.; Harris, Jason B.; Ryan, Edward T.; Qadri, Firdausi (American Society for Microbiology, 2011)The mediators of protective immunity against cholera are currently unknown, but memory B-cell responses may play a central role in facilitating long-term and anamnestic responses against Vibrio cholerae, the cause of ... -
Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults
Alam, Mohammad Murshid; Aktar, Amena; Afrin, Sadia; Rahman, Mohammad Arif; Aktar, Sarmin; Uddin, Taher; Rahman, M. Arifur; Mahbuba, Deena Al; Chowdhury, Fahima; Khan, Ashraful Islam; Bhuiyan, Taufiqur Rahman; Begum, Yasmin Ara; Ryan, Edward T.; Calderwood, Stephen B.; Svennerholm, Ann-Mari; Qadri, Firdausi (Public Library of Science, 2014)Background: Multiple infections with diverse enterotoxigenic E. coli (ETEC) strains lead to broad spectrum protection against ETEC diarrhea. However, the precise mechanism of protection against ETEC infection is still ... -
Antigen-Specific Memory B-Cell Responses to Vibrio cholerae O1 Infection in Bangladesh
Harris, Aaron M.; Bhuiyan, M. Saruar; Chowdhury, Fahima; Khan, Ashraful I.; Hossain, Azim; Kendall, Emily A.; Rahman, Atiqur; LaRocque, Regina C.; Wrammert, Jens; Ryan, Edward T.; Qadri, Firdausi; Calderwood, Stephen B.; Harris, Jason B. (American Society for Microbiology, 2009)Cholera, caused by Vibrio cholerae, is a noninvasive dehydrating enteric disease with a high mortality rate if untreated. Infection with V. cholerae elicits long-term protection against subsequent disease in countries where ... -
Antigen-Specific Memory T Cell Responses after Vaccination with an Oral Killed Cholera Vaccine in Bangladeshi Children and Comparison to Responses in Patients with Naturally Acquired Cholera
Arifuzzaman, Mohammad; Rashu, Rasheduzzaman; Leung, Daniel T.; Hosen, M. Ismail; Bhuiyan, Taufiqur Rahman; Bhuiyan, M. Saruar; Rahman, Mohammad Arif; Khanam, Farhana; Saha, Amit; Charles, Richelle C.; LaRocque, Regina C.; Weil, Ana A.; Clements, John D.; Holmes, Randall K.; Calderwood, Stephen B.; Harris, Jason B.; Ryan, Edward T.; Qadri, Firdausi (American Society for Microbiology, 2012)Young children, older children, and adults develop comparable levels and durations of immunity following cholera. In comparison, young children receiving oral killed cholera vaccines (OCV) develop a lower level and shorter ... -
Biomarkers of Environmental Enteropathy are Positively Associated with Immune Responses to an Oral Cholera Vaccine in Bangladeshi Children
Uddin, Muhammad Ikhtear; Islam, Shahidul; Nishat, Naoshin S.; Hossain, Motaher; Rafique, Tanzeem Ahmed; Rashu, Rasheduzzaman; Hoq, Mohammad Rubel; Zhang, Yue; Saha, Amit; Harris, Jason B.; Calderwood, Stephen B.; Bhuiyan, Taufiqur Rahman; Ryan, Edward T.; Leung, Daniel T.; Qadri, Firdausi (Public Library of Science, 2016)Environmental enteropathy (EE) is a poorly understood condition that refers to chronic alterations in intestinal permeability, absorption, and inflammation, which mainly affects young children in resource-limited settings. ... -
Blood Group, Immunity, and Risk of Infection with Vibrio cholerae in an Area of Endemicity
Harris, Jason B.; Khan, Ashraful I.; LaRocque, Regina C.; Dorer, David J.; Chowdhury, Fahima; Faruque, Abu G.; Sack, David A.; Ryan, Edward T.; Qadri, Firdausi; Calderwood, Stephen B. (American Society for Microbiology, 2005)Individuals with blood group O are more susceptible than other individuals to severe cholera, although the mechanism underlying this association is unknown. To assess the respective roles of both intrinsic host factors and ... -
Cellular and Cytokine Responses to Salmonella enterica Serotype Typhi Proteins in Patients with Typhoid Fever in Bangladesh
Bhuiyan, Saruar; Sayeed, Abu; Khanam, Farhana; Leung, Daniel T.; Rahman Bhuiyan, Taufiqur; Sheikh, Alaullah; Salma, Umme; LaRocque, Regina C.; Harris, Jason B.; Pacek, Marcin; Calderwood, Stephen B.; LaBaer, Joshua; Ryan, Edward T.; Qadri, Firdausi; Charles, Richelle C. (The American Society of Tropical Medicine and Hygiene, 2014)We assessed interferon-gamma (IFN-γ) responses via enzyme-linked immunosorbent spot (ELISPOT) to a number of S. Typhi antigens in samples from humans with S. Typhi bacteremia and typhoid fever in Bangladesh. Compared with ... -
Characterization of Anti-Salmonella enterica Serotype Typhi Antibody Responses in Bacteremic Bangladeshi Patients by an Immunoaffinity Proteomics-Based Technology
Charles, Richelle C.; Sheikh, Alaullah; Krastins, Bryan; Harris, Jason B.; Bhuiyan, M. Saruar; LaRocque, Regina C.; Logvinenko, Tanya; Sarracino, David A.; Kudva, Indira T.; Eisenstein, Jana; Podolsky, Michael J.; Kalsy, Anuj; Brooks, W. Abdullah; Ludwig, Albrecht; John, Manohar; Calderwood, Stephen B.; Qadri, Firdausi; Ryan, Edward T. (American Society for Microbiology, 2010)Salmonella enterica serotype Typhi is the cause of typhoid fever and a human-restricted pathogen. Currently available typhoid vaccines provide 50 to 90% protection for 2 to 5 years, and available practical diagnostic assays ... -
Chemoproteomic profiling of host and pathogen enzymes active in cholera
Hatzios, Stavroula K.; Abel, Sören; Martell, Julianne; Hubbard, Troy; Sasabe, Jumpei; Munera, Diana; Clark, Lars; Bachovchin, Daniel A.; Qadri, Firdausi; Ryan, Edward T.; Davis, Brigid M.; Weerapana, Eranthie; Waldor, Matthew K. (2016)Activity-based protein profiling (ABPP) is a chemoproteomic tool for detecting active enzymes in complex biological systems. We used ABPP to identify secreted bacterial and host serine hydrolases that are active in animals ... -
A Cholera Conjugate Vaccine Containing O-specific Polysaccharide (OSP) of V. cholerae O1 Inaba and Recombinant Fragment of Tetanus Toxin Heavy Chain (OSP:rTTHc) Induces Serum, Memory and Lamina Proprial Responses against OSP and Is Protective in Mice
Sayeed, Md. Abu; Bufano, Meagan Kelly; Xu, Peng; Eckhoff, Grace; Charles, Richelle C.; Alam, Mohammad Murshid; Sultana, Tania; Rashu, Md. Rasheduzzaman; Berger, Amanda; Gonzalez-Escobedo, Geoffrey; Mandlik, Anjali; Bhuiyan, Taufiqur Rahman; Leung, Daniel T.; LaRocque, Regina C.; Harris, Jason B.; Calderwood, Stephen B.; Qadri, Firdausi; Vann, W. F.; Kováč, Pavol; Ryan, Edward T. (Public Library of Science, 2015)Background: Vibrio cholerae is the cause of cholera, a severe watery diarrhea. Protection against cholera is serogroup specific. Serogroup specificity is defined by the O-specific polysaccharide (OSP) component of ... -
Circulating Mucosal Associated Invariant T Cells Are Activated in Vibrio cholerae O1 Infection and Associated with Lipopolysaccharide Antibody Responses
Leung, Daniel T.; Bhuiyan, Taufiqur R.; Nishat, Naoshin S.; Hoq, Mohammad Rubel; Aktar, Amena; Rahman, M. Arifur; Uddin, Taher; Khan, Ashraful I.; Chowdhury, Fahima; Charles, Richelle C.; Harris, Jason B.; Calderwood, Stephen B.; Qadri, Firdausi; Ryan, Edward T. (Public Library of Science, 2014)Background: Mucosal Associated Invariant T (MAIT) cells are innate-like T cells found in abundance in the intestinal mucosa, and are thought to play a role in bridging the innate-adaptive interface. Methods: We measured ... -
Comparative Proteomic Analysis of the PhoP Regulon in Salmonella enterica Serovar Typhi Versus Typhimurium
Lebrun, Lauren M.; Sheikh, Alaullah; Logvinenko, Tanya; Tarique, Abdullah; Krastins, Bryan; Qadri, Firdausi; Charles, Richelle Candice; Harris, Jason B.; Chase, Michael Richard; Larocque, Regina Celes; Rollins, Sean McKenzie; Hohmann, Elizabeth Louise; Rosenberg, Ian Morley; Sarracino, David A.; Calderwood, Stephen Beaven; Ryan, Edward Thomas (Public Library of Science, 2009)Background: S. Typhi, a human-restricted Salmonella enterica serovar, causes a systemic intracellular infection in humans (typhoid fever). In comparison, S. Typhimurium causes gastroenteritis in humans, but causes a systemic ... -
Comparative Proteomic Analysis Reveals Activation of Mucosal Innate Immune Signaling Pathways during Cholera
Ellis, Crystal; LaRocque, Regina; Uddin, Taher; Krastins, Bryan; Mayo-Smith, Leslie; Sarracino, David; Karlsson, Elinor; Rahman, Atiqur; Shirin, Tahmina; Bhuiyan, Taufiqur; Chowdhury, Fahima; Khan, Ashraful Islam; Ryan, Edward; Calderwood, Stephen; Qadri, Firdausi; Harris, Jason (American Society for Microbiology, 2015)Vibrio cholerae O1 is a major cause of acute watery diarrhea in over 50 countries. Evidence suggests that V. cholerae O1 may activate inflammatory pathways, and a recent study of a Bangladeshi population showed that variants ... -
Comparison of Immune Responses to the O-Specific Polysaccharide and Lipopolysaccharide of Vibrio cholerae O1 in Bangladeshi Adult Patients with Cholera
Johnson, Russell A.; Uddin, Taher; Aktar, Amena; Mohasin, M.; Alam, Mohammad Murshid; Chowdhury, Fahima; Harris, Jason B.; LaRocque, Regina C.; Bufano, Meagan Kelly; Yu, Yanan; Wu-Freeman, Ying; Leung, Daniel T.; Sarracino, David; Krastins, Bryan; Charles, Richelle C.; Xu, Peng; Kováč, Pavol; Calderwood, Stephen B.; Qadri, Firdausi; Ryan, Edward T. (American Society for Microbiology, 2012)Immunity against Vibrio cholerae O1 is serogroup specific, and serogrouping is defined by the O-specific polysaccharide (OSP) part of lipopolysaccharide (LPS). Despite this, human immune responses to V. cholerae OSP have ... -
Comparison of Memory B Cell, Antibody-Secreting Cell, and Plasma Antibody Responses in Young Children, Older Children, and Adults with Infection Caused by Vibrio cholerae O1 El Tor Ogawa in Bangladesh
Leung, Daniel T.; Rahman, Mohammad Arif; Mohasin, M.; Riyadh, M. Asrafuzzaman; Patel, Sweta M.; Alam, Mohammad Murshid; Chowdhury, Fahima; Khan, Ashraful Islam; Kalivoda, Eric J.; Aktar, Amena; Bhuiyan, M. Saruar; LaRocque, Regina C.; Harris, Jason B.; Calderwood, Stephen B.; Qadri, Firdausi; Ryan, Edward T. (American Society for Microbiology, 2011)Children bear a large component of the global burden of cholera. Despite this, little is known about immune responses to cholera in children, especially those under 5 years of age. Cholera vaccine studies have demonstrated ...