Browsing Harvard Medical School by Title
Now showing items 15033-15052 of 18428
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RNA Aptamer Probes as Optical Imaging Agents for the Detection of Amyloid Plaques
(Public Library of Science, 2014)Optical imaging using multiphoton microscopy and whole body near infrared imaging has become a routine part of biomedical research. However, optical imaging methods rely on the availability of either small molecule reporters ... -
The RNA Binding Protein ESRP1 Fine-Tunes the Expression of Pluripotency-Related Factors in Mouse Embryonic Stem Cells
(Public Library of Science, 2013)In pluripotent stem cells, there is increasing evidence for crosstalk between post-transcriptional and transcriptional networks, offering multifold steps at which pluripotency can be controlled. In addition to well-studied ... -
RNA Catalysis in Model Protocell Vesicles
(American Chemical Society, 2005)We are engaged in a long-term effort to synthesize chemical systems capable of Darwinian evolution, based on the encapsulation of self-replicating nucleic acids in self-replicating membrane vesicles. Here, we address the ... -
RNA Expression Patterns in Serum Microvesicles from Patients with Glioblastoma Multiforme and Controls
(BioMed Central, 2012)Background: RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients. ... -
RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells
(Springer Science and Business Media LLC, 2020-03-30)The availability of nucleotides has a direct impact on transcription. Inhibition of dihydroorate dehydrogenase (DHODH) with leflunomide impacts nucleotide pools by reducing pyrimidine levels. Leflunomide abrogates effective ... -
An RNA Interference Lethality Screen of the Human Druggable Genome to Identify Molecular Vulnerabilities in Epithelial Ovarian Cancer
(Public Library of Science, 2012)Targeted therapies have been used to combat many tumor types; however, few have effectively improved the overall survival in women with epithelial ovarian cancer, begging for a better understanding of this deadly disease ... -
RNA Interference Screening in Drosophila Primary Cells for Genes Involved in Muscle Assembly and Maintenance
(The Company of Biologists, 2008-04-15)To facilitate the genetic analysis of muscle assembly and maintenance, we have developed a method for efficient RNA interference (RNAi) in Drosophila primary cells using double-stranded RNAs (dsRNAs). First, using molecular ... -
RNA Sequence Analysis of Human Huntington Disease Brain Reveals an Extensive Increase in Inflammatory and Developmental Gene Expression
(Public Library of Science, 2015)Huntington’s Disease (HD) is a devastating neurodegenerative disorder that is caused by an expanded CAG trinucleotide repeat in the Huntingtin (HTT) gene. Transcriptional dysregulation in the human HD brain has been ... -
RNA sequence analysis reveals macroscopic somatic clonal expansion across normal tissues
(American Association for the Advancement of Science (AAAS), 2019-06-06)How somatic mutations accumulate in normal cells is poorly understood. A comprehensive analysis of RNA-sequencing data from ~6,700 samples across 29 normal tissues reveals multiple somatic variants, demonstrating that ... -
RNA Sequence Determinants of a Coupled Termination-Reinitiation Strategy for Downstream Open Reading Frame Translation in Helminthosporium victoriae Virus 190S and Other Victoriviruses (Family Totiviridae)
(American Society for Microbiology, 2011)The genome-length, dicistronic mRNA of the double-stranded RNA fungal virus Helminthosporium victoriae virus 190S (genus Victorivirus, family Totiviridae) contains two long open reading frames (ORFs) that overlap in the ... -
RNA sequencing from neural ensembles activated during fear conditioning in the mouse temporal association cortex
(Nature Publishing Group, 2016)The stable formation of remote fear memories is thought to require neuronal gene induction in cortical ensembles that are activated during learning. However, the set of genes expressed specifically in these activated ... -
RNA sequencing of cancer reveals novel splicing alterations
(Nature Publishing Group, 2013)Breast cancer transcriptome acquires a myriad of regulation changes, and splicing is critical for the cell to “tailor-make” specific functional transcripts. We systematically revealed splicing signatures of the three most ... -
RNA Sequencing of Pancreatic Circulating Tumour Cells Implicates WNT Signaling in Metastasis
(2012)Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. While these cells are extraordinarily rare, they may identify cellular pathways contributing ... -
RNA Sequencing Reveals Differential Expression of Mitochondrial and Oxidation Reduction Genes in the Long-Lived Naked Mole-Rat When Compared to Mice
(Public Library of Science (PLoS), 2011-11-03)The naked mole-rat (Heterocephalus glaber) is a long-lived, cancer resistant rodent and there is a great interest in identifying the adaptations responsible for these and other of its unique traits. We employed RNA sequencing ... -
RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities
(National Academy of Sciences, 2012)With rising rates of drug-resistant infections, there is a need for diagnostic methods that rapidly can detect the presence of pathogens and reveal their susceptibility to antibiotics. Here we propose an approach to ... -
RNA Stimulates Aurora B Kinase Activity during Mitosis
(Public Library of Science, 2014)Accurate chromosome segregation is essential for cell viability. The mitotic spindle is crucial for chromosome segregation, but much remains unknown about factors that regulate spindle assembly. Recent work implicates RNA ... -
RNA Transfer from Poliovirus 135S Particles across Membranes Is Mediated by Long Umbilical Connectors
(American Society for Microbiology, 2013)During infection, the binding of poliovirus to its cell surface receptor at 37 degrees C triggers an expansion of the virus in which internal polypeptides that bind to membranes are externalized. Subsequently, in a poorly ... -
RNA Velocity of Single Cells
(Springer Science and Business Media LLC, 2018-08)RNA abundance is a powerful indicator of the state of individual cells. Single-cell RNA sequencing can reveal RNA abundance with high quantitative accuracy, sensitivity and throughput1. However, this approach captures only ... -
RNA-associated protein 55 (RAP55) localizes to mRNA processing bodies and stress granules
(Cold Spring Harbor Laboratory Press, 2006)The mRNA processing body (P-body) is a cellular structure that has an important role in mRNA degradation. P-bodies have also been implicated in RNAi-mediated post-transcriptional gene silencing. The objective of this study ... -
RNA-Seq of the Caribbean reef-building coral Orbicella faveolata (Scleractinia-Merulinidae) under bleaching and disease stress expands models of coral innate immunity
(PeerJ Inc., 2016)Climate change-driven coral disease outbreaks have led to widespread declines in coral populations. Early work on coral genomics established that corals have a complex innate immune system, and whole-transcriptome gene ...