Browsing Harvard Medical School by Title
Now showing items 8803-8822 of 18428
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In Silico Promoter Analysis can Predict Genes of Functional Relevance in Cell Proliferation: Validation in a Colon Cancer Model
(Sage, 2007-02)Specific combinations of transcription-factor binding sites in the promoter regions of genes regulate gene expression, and thus key functional processes in cells. Analysis of such promoter regions in specific functional ... -
in silico Surveillance: evaluating outbreak detection with simulation models
(BioMed Central, 2013)Background: Detecting outbreaks is a crucial task for public health officials, yet gaps remain in the systematic evaluation of outbreak detection protocols. The authors’ objectives were to design, implement, and test a ... -
In situ localized amplification and contact replication of many individual DNA molecules
(Oxford University Press (OUP), 1999-12-15)We describe a method to clone and amplify DNA by performing the polymerase chain reaction (PCR) in a thin polyacrylamide film poured on a glass microscope slide. The polyacrylamide matrix retards the diffusion of the linear ... -
In situ regeneration of bioactive coatings enabled by an evolved Staphylococcus aureus sortase A
(Nature Publishing Group, 2016)Surface immobilization of bioactive molecules is a central paradigm in the design of implantable devices and biosensors with improved clinical performance capabilities. However, in vivo degradation or denaturation of surface ... -
In situ single cell analysis identifies heterogeneity for PIK3CA mutation and HER2 amplification in HER2+ breast cancer
(2015)Detection of minor genetically distinct subpopulations within tumors is a key challenge in cancer genomics. Here we report STAR-FISH (Specific-To-Allele PCR – FISH), a novel method for the combined detection of single ... -
In the Early Postpartum Period, Parents are Interested in Newborn Genomic Testing
(2014)Purpose We surveyed parents to ascertain interest in newborn genomic testing and determine whether these queries would provoke refusal of conventional newborn screening (NBS). Methods: After brief genetics orientation, ... -
In the Information Age, do dementia caregivers get the information they need? Semi-structured interviews to determine informal caregivers’ education needs, barriers, and preferences
(BioMed Central, 2016)Background: Most patients with dementia or cognitive impairment receive care from family members, often untrained for this challenging role. Caregivers may not access publicly available caregiving information, and caregiver ... -
In Utero and Early-Life Conditions and Adult Health and Disease
(New England Journal of Medicine (NEJM/MMS), 2008) -
In utero gene therapy rescues microcephaly caused by Pqbp1-hypofunction in neural stem progenitor cells
(Nature Publishing Group, 2015)Human mutations in PQBP1, a molecule involved in transcription and splicing, result in a reduced but architecturally normal brain. Examination of a conditional Pqbp1-knockout (cKO) mouse with microcephaly failed to reveal ... -
In Vitro and in Vivo Gene Delivery using a Lentiviral Vector
(BioMed Central, 2001) -
In Vitro and In Vivo Models of Staphylococcus aureus Endophthalmitis Implicate Specific Nutrients in Ocular Infection
(Public Library of Science, 2014)Purpose To define global transcriptional responses of Staphylococcus aureus and its codY mutant (CodY is a transcription regulator of virulence and metabolic genes in response to branched-chain amino acids) when growing ... -
In vitro and in vivo studies of VISTA: a B7 family immune checkpoint receptor
(2021-06-25)Immunotherapy has become an established pillar of cancer treatment, in large part owing to the success of blocking the programmed cell death protein 1 (PD-1)/ programmed death-ligand 1 (PD- L1) immune checkpoint pathway. ... -
In vitro and in vivo studies on the importance of the soluble guanylyl cyclase a1 subunit in penile erection
(Springer Nature, 2010)Purpose: Soluble guanylyl cyclase (sGC), which plays a pivotal role in penile erection, is a heterodimer build up by an α and a β subunit. For both subunits two isoforms have been characterized, but only the sGCα1β1 and ... -
In Vitro Antimicrobial Activity of Wall Teichoic Acid Biosynthesis Inhibitors against Staphylococcus aureus Isolates
(American Society for Microbiology, 2010)Staphylococcus aureus is the leading cause of invasive and superficial human infections, is increasingly antibiotic resistant, and is therefore the target for the development of new antimicrobials. Compounds (1835F03 and ... -
In vitro characterization of the human segmentation clock
(Springer Science and Business Media LLC, 2020-01-08)The vertebral column is characterized by the periodic arrangement of vertebrae along the anterior-posterior (AP) axis. This segmental or metameric organization is established early in embryogenesis when pairs of embryonic ... -
In vitro evaluation of bi-layer silk fibroin scaffolds for gastrointestinal tissue engineering
(SAGE Publications, 2014)Silk fibroin scaffolds were investigated for their ability to support attachment, proliferation, and differentiation of human gastrointestinal epithelial and smooth muscle cell lines in order to ascertain their potential ... -
In vitro evaluation of digestive and endolysosomal enzymes to cleave CML-modified Ara h 1 peptides
(John Wiley & Sons, Ltd, 2015)Ara h 1 is a major peanut allergen. Processing-induced modifications may modulate the allergenic potency of Ara h 1. Carboxymethyl lysine (CML) modifications are a commonly described nonenzymatic modification on food ... -
In vitro modeling to determine mutation specificity of EGFR tyrosine kinase inhibitors against clinically relevant EGFR mutants in non-small-cell lung cancer
(Impact Journals LLC, 2015)EGFR mutated lung cancer accounts for a significant subgroup of non-small-cell lung cancer (NSCLC). Over the last decade, multiple EGFR tyrosine kinase inhibitors (EGFR-TKIs) have been developed to target mutated EGFR. ... -
In vitro models of cancer stem cells and clinical applications
(BioMed Central, 2016)Cancer cells, stem cells and cancer stem cells have for a long time played a significant role in the biomedical sciences. Though cancer therapy is more effective than it was a few years ago, the truth is that still none ... -
In Vitro Priming Recapitulates In Vivo HIV-1 Specific T Cell Responses, Revealing Rapid Loss of Virus Reactive CD4+ T Cells in Acute HIV-1 Infection
(Public Library of Science, 2009)Background: The requirements for priming of HIV-specific T cell responses initially seen in infected individuals remain to be defined. Activation of T cell responses in lymph nodes requires cell-cell contact between T cells ...