Browsing Harvard Medical School by Title
Now showing items 750-769 of 18428
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An autoregulatory loop controls peroxisome proliferator-activated receptor γ coactivator 1α expression in muscle
(National Academy of Sciences, 2003) -
An Escherichia coli O157-Specific Engineered Pyocin Prevents and Ameliorates Infection by E. coli O157:H7 in an Animal Model of Diarrheal Disease
(American Society for Microbiology, 2011)AvR2-V10.3 is an engineered R-type pyocin that specifically kills Escherichia coli O157, an enteric pathogen that is a major cause of food-borne diarrheal disease. New therapeutics to counteract E. coli O157 are needed, ... -
An essential role for RPE-derived soluble VEGF in the maintenance of the choriocapillaris
(National Academy of Sciences, 2009)Clinical and experimental observations indicate a role for VEGF secreted by the retinal pigment epithelium (RPE) in the maintenance of the choriocapillaris (CC). VEGF in mice is produced as three isoforms, VEGF120, VEGF164, ... -
An Externalized Polypeptide Partitions between Two Distinct Sites on Genome-Released Poliovirus Particles
(American Society for Microbiology, 2011)During cell entry, native poliovirus (160S) converts to a cell-entry intermediate (135S) particle, resulting in the externalization of capsid proteins VP4 and the amino terminus of VP1 (residues 1 to 53). Externalization ... -
An in Trans Interaction at the Interface of the Helicase and Primase Domains of the Hexameric Gene 4 Protein of Bacteriophage T7 Modulates Their Activities
(American Society for Biochemistry and Molecular Biology, 2009)DNA helicase and primase are essential for DNA replication. The helicase unwinds the DNA to provide single-stranded templates for DNA polymerase. The primase catalyzes the synthesis of oligoribonucleotides for the initiation ... -
An in Vitro RNA Synthesis Assay for Rabies Virus Defines Ribonucleoprotein Interactions Critical for Polymerase Activity
(American Society for Microbiology, 2017)We report an in vitro RNA synthesis assay for the RNA-dependent RNA polymerase (RdRP) of rabies virus (RABV). We expressed RABV large polymerase protein (L) in insect cells from a recombinant baculovirus vector and the ... -
An in-tumor genetic screen reveals that the BET bromodomain protein, BRD4, is a potential therapeutic target in ovarian carcinoma
(National Academy of Sciences, 2015)High-grade serous ovarian carcinoma (HGSOC) is the most common and aggressive form of epithelial ovarian cancer, for which few targeted therapies exist. To search for new therapeutic target proteins, we performed an in ... -
An Interaction between DNA Polymerase and Helicase Is Essential for the High Processivity of the Bacteriophage T7 Replisome
(American Society for Biochemistry and Molecular Biology, 2012)Synthesis of the leading DNA strand requires the coordinated activity of DNA polymerase and DNA helicase, whereas synthesis of the lagging strand involves interactions of these proteins with DNA primase. We present the ... -
An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants
(National Academy of Sciences, 2006)Random transposon insertion libraries have proven invaluable in studying bacterial genomes. Libraries that approach saturation must be large, with multiple insertions per gene, making comprehensive genome-wide scanning ... -
An RNA-binding Protein, Lin28, Recognizes and Remodels G-quartets in the MicroRNAs (miRNAs) and mRNAs It Regulates
(American Society for Biochemistry and Molecular Biology, 2015)Lin28 is an evolutionarily conserved RNA-binding protein that inhibits processing of pre-let-7 microRNAs (miRNAs) and regulates translation of mRNAs that control developmental timing, pluripotency, metabolism, and ... -
An unusual internal ribosome entry site in the herpes simplex virus thymidine kinase gene
(National Academy of Sciences, 2005)We have investigated a herpes simplex virus mutant that expresses low levels of thymidine kinase (TK), a phenotype associated with drug resistance and pathogenicity, despite a single-base deletion in the gene. Using a ... -
Anaemia, prenatal iron use, and risk of adverse pregnancy outcomes: systematic review and meta-analysis
(BMJ Publishing Group Ltd., 2013)Objectives: To summarise evidence on the associations of maternal anaemia and prenatal iron use with maternal haematological and adverse pregnancy outcomes; and to evaluate potential exposure-response relations of dose of ... -
Analgesia and unwanted benzodiazepine effects in point-mutated mice expressing only one benzodiazepine-sensitive GABAA receptor subtype
(Nature Publishing Group, 2015)Agonists at the benzodiazepine-binding site of GABAA receptors (BDZs) enhance synaptic inhibition through four subtypes (α1, α2, α3 and α5) of GABAA receptors (GABAAR). When applied to the spinal cord, they alleviate ... -
Analyses of Human–Chimpanzee Orthologous Gene Pairs to Explore Evolutionary Hypotheses of Aging
(Elsevier BV, 2007-05)Compared to chimpanzees (Pan troglodytes), the onset of aging appears to be delayed in the human species. Herein, we studied human-chimpanzee orthologous gene pairs to investigate the selective forces acting on genes ... -
Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors
(Nature Pub. Group, 2015)Decoding heterogeneity of pluripotent stem cell (PSC)-derived neural progeny is fundamental for revealing the origin of diverse progenitors, for defining their lineages, and for identifying fate determinants driving ... -
Analysis and Application of European Genetic Substructure Using 300 K SNP Information
(Public Library of Science, 2008)European population genetic substructure was examined in a diverse set of >1,000 individuals of European descent, each genotyped with >300 K SNPs. Both STRUCTURE and principal component analyses (PCA) showed the largest ... -
An Analysis and Validation Pipeline for Large-Scale RNAi-Based Screens
(Springer Science and Business Media LLC, 2013-01-16)Large-scale RNAi-based screens are a major technology, but require adequate prioritization and validation of candidate genes from the primary screen. In this work, we performed a large-scale pooled shRNA screen in mouse ... -
Analysis Method and Experimental Conditions Affect Computed Circadian Phase from Melatonin Data
(Public Library of Science, 2012)Accurate determination of circadian phase is necessary for research and clinical purposes because of the influence of the master circadian pacemaker on multiple physiologic functions. Melatonin is presently the most accurate ... -
Analysis of 6,515 exomes reveals a recent origin of most human protein-coding variants
(2012)Establishing the age of each mutation segregating in contemporary human populations is important to fully understand our evolutionary history1,2 and will help facilitate the development of new approaches for disease gene ... -
Analysis of a Mouse Skin Model of Tuberous Sclerosis Complex
(Public Library of Science, 2016)Tuberous Sclerosis Complex (TSC) is an autosomal dominant tumor suppressor gene syndrome in which patients develop several types of tumors, including facial angiofibroma, subungual fibroma, Shagreen patch, angiomyolipomas, ...