Browsing Harvard Medical School by Keyword "biotechnology"

Now showing items 1-20 of 29

    • Blood Banking in Living Droplets 

      Samot, Josh; Moon, Sangjun; Shao, Lei; Zhang, Xiaohui; Xu, Feng; Song, YoungSeok; Keles, Hasan Onur; Matloff, Laura; Markel, Jordan; Demirci, Utkan (Public Library of Science, 2011)
      Blood banking has a broad public health impact influencing millions of lives daily. It could potentially benefit from emerging biopreservation technologies. However, although vitrification has shown advantages over traditional ...

    • Broad Spectrum Antiangiogenic Treatment for Ocular Neovascular Diseases 

      Benny, Ofra; Nakai, Kei; Yoshimura, Takeru; Bazinet, Lauren; Akula, James Daniel; Nakao, Shintaro; Hafezi-Moghadam, Ali; Panigrahy, Dipak; Pakneshan, Pouya; D'Amato, Robert John (Public Library of Science, 2010)
      Pathological neovascularization is a hallmark of late stage neovascular (wet) age-related macular degeneration (AMD) and the leading cause of blindness in people over the age of 50 in the western world. The treatments focus ...

    • Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices 

      Butler, Kathryn Lee; Ambravaneswaran, Vijayakrishnan; Agrawal, Nitin; Bilodeau, Maryelizabeth; Toner, Mehmet; Tompkins, Ronald Gary; Fagan, Shawn P.; Irimia, Daniel (Public Library of Science, 2010)
      Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies ...

    • Concept, Design and Implementation of a Cardiovascular Gene-centric 50 K SNP Array for Large-scale Genomic Association Studies 

      Keating, Brendan J.; Tischfield, Sam; Murray, Sarah S.; Bhangale, Tushar; Price, Thomas S.; Glessner, Joseph T.; Galver, Luana; Barrett, Jeffrey C.; Grant, Struan F. A.; Farlow, Deborah N.; Chandrupatla, Hareesh R.; Ajmal, Saad; Papanicolaou, George J.; Guo, Yiran; Li, Mingyao; DerOhannessian, Stephanie; Bailey, Swneke D.; Montpetit, Alexandre; Edmondson, Andrew C.; Taylor, Kent; Gai, Xiaowu; Wang, Susanna S.; Fornage, Myriam; Shaikh, Tamim; Groop, Leif; Boehnke, Michael; Hall, Alistair S.; Hattersley, Andrew T.; Frackelton, Edward; Patterson, Nick; Chiang, Charleston W. K.; Kim, Cecelia E.; Fabsitz, Richard R.; Ouwehand, Willem; Munroe, Patricia; Caulfield, Mark; Drake, Thomas; Boerwinkle, Eric; Whitehead, A. Stephen; Cappola, Thomas P.; Samani, Nilesh J.; Lusis, A. Jake; Schadt, Eric; Wilson, James G.; Koenig, Wolfgang; McCarthy, Mark I.; Kathiresan, Sekar; Gabriel, Stacey B.; Hakonarson, Hakon; Anand, Sonia S.; Reilly, Muredach; Engert, James C.; Nickerson, Deborah A.; Rader, Daniel J.; FitzGerald, Garret A.; Reitsma, Pieter H.; Hansen, Mark; de Bakker, Paul I Wen; Price, Alkes; Reich, David Emil; Hirschhorn, Joel Naom (Public Library of Science, 2008)
      A wealth of genetic associations for cardiovascular and metabolic phenotypes in humans has been accumulating over the last decade, in particular a large number of loci derived from recent genome wide association studies ...

    • Control and Manipulation of Pathogens with an Optical Trap for Live Cell Imaging of Intercellular Interactions 

      Tam, Jenny M.; Castro, Carlos E.; Heath, Robert John William; Cardenas, Michael L.; Xavier, Ramnik; Lang, Matthew J.; Vyas, Jatin Mahesh (Public Library of Science, 2010)
      The application of live cell imaging allows direct visualization of the dynamic interactions between cells of the immune system. Some preliminary observations challenge long-held beliefs about immune responses to microorganisms; ...

    • Drop-on-Demand Single Cell Isolation and Total RNA Analysis 

      Moon, Sangjun; Kim, Yun-Gon; Dong, Lingsheng; Lombardi, Michael; Haeggstrom, Edward Olof; Jensen, Roderick V.; Hsiao, Li-Li; Demirci, Utkan (Public Library of Science, 2011)
      Technologies that rapidly isolate viable single cells from heterogeneous solutions have significantly contributed to the field of medical genomics. Challenges remain both to enable efficient extraction, isolation and ...

    • Evaluation of Silk Biomaterials in Combination with Extracellular Matrix Coatings for Bladder Tissue Engineering with Primary and Pluripotent Cells 

      Franck, Debra; Gil, Eun Seok; Adam, Rosalyn Mare; Kaplan, David L.; Chung, Yeun Goo; Estrada, Carlos R.; Mauney, Joshua Robert (Public Library of Science, 2013)
      Silk-based biomaterials in combination with extracellular matrix (ECM) coatings were assessed as templates for cell-seeded bladder tissue engineering approaches. Two structurally diverse groups of silk scaffolds were ...

    • Formation of Toxic Oligomeric α-Synuclein Species in Living Cells 

      Outeiro, Tiago Fleming; Putcha, Preeti; Tetzlaff, Julie E.; Spoelgen, Robert; Koker, Mirjam; Carvalho, Filipe; Hyman, Bradley Theodore; McLean, Pamela June (Public Library of Science, 2008)
      Background: Misfolding, oligomerization, and fibrillization of α-synuclein are thought to be central events in the onset and progression of Parkinson's disease (PD) and related disorders. Although fibrillar α-synuclein is ...

    • A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis 

      Murthy, Tal; Raphael, Jacob; Moreira, Donna; Kelley, Fontina; Taycher, Elena; Brizuela, Leonardo; LaBaer, Joshua; Petrosino, Joseph; Rolfs, Andreas; Hu, Yanhui; McCarron, Seamus; Zuo, Dongmei; Shi, Zhenwei; Jepson, Daniel Ashley; Mohr, Stephanie; Fernandez, Mauricio J. (Public Library of Science, 2007)
      The rapid development of new technologies for the high throughput (HT) study of proteins has increased the demand for comprehensive plasmid clone resources that support protein expression. These clones must be full-length, ...

    • Guarding Embryo Development of Zebrafish by Shell Engineering: A Strategy to Shield Life from Ozone Depletion 

      Tang, Yanyan; Pan, Haihua; Xu, Xurong; Tang, Ruikang; Wang, Ben; Liu, Peng (Public Library of Science, 2010)
      Background: The reduced concentration of stratospheric ozone results in an increased flux of biologically damaging mid-ultraviolet radiation (UVB, 280 to 320 nm) reaching earth surfaces. Environmentally relevant levels of ...

    • A High-Throughput Screen Identifies a New Natural Product with Broad-Spectrum Antibacterial Activity 

      Ymele-Leki, Patrick; Cao, Shugeng; Sharp, Jared D'Alonzo; Lambert, Kathleen G; McAdam, Alexander John; Husson, Robert N; Tamayo, Giselle; Clardy, Jon C.; Watnick, Paula Ivonne (Public Library of Science, 2012)
      Due to the inexorable invasion of our hospitals and communities by drug-resistant bacteria, there is a pressing need for novel antibacterial agents. Here we report the development of a sensitive and robust but low-tech and ...

    • Identification and Rational Redesign of Peptide Ligands to CRIP1, A Novel Biomarker for Cancers 

      Hao, Jihua; Serohijos, Adrian; Newton, Gail; Tassone, Gina; Wang, Zuncai; Sgroi, Dennis Charles; Dokholyan, Nikolay V.; Basilion, James P. (Public Library of Science, 2008)
      Cysteine-rich intestinal protein 1 (CRIP1) has been identified as a novel marker for early detection of cancers. Here we report on the use of phage display in combination with molecular modeling to identify a high-affinity ...

    • Insulin-Like Growth Factors Promote Vasculogenesis in Embryonic Stem Cells 

      Piecewicz, Stephanie M.; Pandey, Ambarish; Roy, Bhaskar; Hua Xiang, Soh; Zetter, Bruce Robert; Sengupta, Shiladitya (Public Library of Science, 2012)
      The ability of embryonic stem cells to differentiate into endothelium and form functional blood vessels has been well established and can potentially be harnessed for therapeutic angiogenesis. However, after almost two ...

    • Integrated Proteomic Analysis of Human Cancer Cells and Plasma from Tumor Bearing Mice for Ovarian Cancer Biomarker Discovery 

      Pitteri, Sharon J.; JeBailey, Lellean; Faça, Vitor M.; Thorpe, Jason D.; Silva, Melissa A.; Ireton, Reneé C.; Horton, Marc B.; Pruitt, Liese C.; Urban, Nicole; Hanash, Samir M.; Nordheim, Alfred; Wang, Hong; Zhang, Qing; Cheng, Yu-Kang; Dinulescu, Daniela M (Public Library of Science, 2009)
      Background: The complexity of the human plasma proteome represents a substantial challenge for biomarker discovery. Proteomic analysis of genetically engineered mouse models of cancer and isolated cancer cells and cell ...

    • Integrative Genomic Data Mining for Discovery of Potential Blood-Borne Biomarkers for Early Diagnosis of Cancer 

      Yang, Yongliang; Iyer, Lakshmanan K.; Adelstein, S. James; Kassis, Amin Iskandar; Hofmann, Oliver Marc; Pospisil, Pavel (Public Library of Science, 2008)
      Background: With the arrival of the postgenomic era, there is increasing interest in the discovery of biomarkers for the accurate diagnosis, prognosis, and early detection of cancer. Blood-borne cancer markers are favored ...

    • Lentivirus Display: Stable Expression of Human Antibodies on the Surface of Human Cells and Virus Particles 

      Taube, Ran; Zhu, Quan Karen; Xu, Chen; Diaz-Griffero, Felipe; Sui, Jianhua; Kamau, Erick; Dwyer, Markryan; Aird, Daniel; Marasco, Wayne A. (Public Library of Science, 2008)
      Background: Isolation of human antibodies using current display technologies can be limited by constraints on protein expression, folding and post-translational modifications. Here we describe a discovery platform that ...

    • Living Bacterial Sacrificial Porogens to Engineer Decellularized Porous Scaffolds 

      Xu, Feng; Sridharan, BanuPriya; Durmus, Naside Gozde; Wang, Shuqi; Yavuz, Ahmet Sinan; Gurkan, Umut Atakan; Demirci, Utkan (Public Library of Science, 2011)
      Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative ...

    • Mesenchymal Stem Cell-Derived Molecules Reverse Fulminant Hepatic Failure 

      Parekkadan, Biju; van Poll, Daan; Suganuma, Kazuhiro; Carter, Edward Albert; Tilles, Arno W.; Yarmush, Martin Leon (Public Library of Science, 2007)
      Modulation of the immune system may be a viable alternative in the treatment of fulminant hepatic failure (FHF) and can potentially eliminate the need for donor hepatocytes for cellular therapies. Multipotent bone ...

    • Metabolic Profiling Based Quantitative Evaluation of Hepatocellular Metabolism in Presence of Adipocyte Derived Extracellular Matrix 

      Sharma, Nripen S.; Nagrath, Deepak; Yarmush, Martin Leon (Public Library of Science, 2011)
      The elucidation of the effect of extracellular matrices on hepatocellular metabolism is critical to understand the mechanism of functional upregulation. We have developed a system using natural extracellular matrices ...

    • A Microchip CD4 Counting Method for HIV Monitoring in Resource-Poor Settings 

      Christodoulides, Nicolaos; Floriano, Pierre N; Graham, Susan; Mohanty, Sanghamitra; Dixon, Meredith; Hsiang, Mina; Peter, Trevor; Zavahir, Shabnam; Romanovicz, Dwight; Bernard, Bruce; Goodey, Adrian P; McDevitt, John T; Rodriguez, William Richard; Thior, Ibou; Walker, Bruce David (Public Library of Science, 2005)
      Background: More than 35 million people in developing countries are living with HIV infection. An enormous global effort is now underway to bring antiretroviral treatment to at least 3 million of those infected. While drug ...