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CRISPR/Cas9-Mediated Phage Resistance Is Not Impeded by the DNA Modifications of Phage T4
(Public Library of Science, 2014)
Bacteria rely on two known DNA-level defenses against their bacteriophage predators: restriction-modification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) systems. Certain ...
Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases
(Public Library of Science, 2012)
Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination. Previous studies have suggested that endogenous ...
Simulating Serial-Target Antibacterial Drug Synergies Using Flux Balance Analysis
(Public Library of Science, 2016)
Flux balance analysis (FBA) is an increasingly useful approach for modeling the behavior of metabolic systems. However, standard FBA modeling of genetic knockouts cannot predict drug combination synergies observed between ...