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Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
(Oxford University Press, 2013)
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide adaptive immunity against invading foreign nucleic ...
Using Bacteria to Determine Protein Kinase Specificity and Predict Target Substrates
(Public Library of Science, 2012)
The identification of protein kinase targets remains a significant bottleneck for our understanding of signal transduction in normal and diseased cellular states. Kinases recognize their substrates in part through sequence ...
Manipulating Replisome Dynamics to Enhance Lambda Red-Mediated Multiplex Genome Engineering
(Oxford University Press, 2012)
Disrupting the interaction between primase and helicase in Escherichia coli increases Okazaki fragment (OF) length due to less frequent primer synthesis. We exploited this feature to increase the amount of ssDNA at the ...
On the design of clone-based haplotyping
(BioMed Central, 2013)
Background: Haplotypes are important for assessing genealogy and disease susceptibility of individual genomes, but are difficult to obtain with routine sequencing approaches. Experimental haplotype reconstruction based on ...
Mutants of Cre recombinase with improved accuracy
(2013)
Despite rapid advances in genome engineering technologies, inserting genes into precise locations in the human genome remains an outstanding problem. It has been suggested that site-specific recombinases can be adapted ...
Harvard Personal Genome Project: lessons from participatory public research
(BioMed Central, 2014)
Background: Since its initiation in 2005, the Harvard Personal Genome Project has enrolled thousands of volunteers interested in publicly sharing their genome, health and trait data. Because these data are highly identifiable, ...
Rational optimization of tolC as a powerful dual selectable marker for genome engineering
(Oxford University Press, 2014)
Selection has been invaluable for genetic manipulation, although counter-selection has historically exhibited limited robustness and convenience. TolC, an outer membrane pore involved in transmembrane transport in E. coli, ...
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
(2013)
Prokaryotic type II CRISPR-Cas systems can be adapted to enable targeted genome modifications across a range of eukaryotes.1–7. Here we engineer this system to enable RNA-guided genome regulation in human cells by tethering ...
CRISPR/Cas9-Mediated Phage Resistance Is Not Impeded by the DNA Modifications of Phage T4
(Public Library of Science, 2014)
Bacteria rely on two known DNA-level defenses against their bacteriophage predators: restriction-modification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) systems. Certain ...
A New Dawn for Industrial Photosynthesis
(Springer Netherlands, 2011)
Several emerging technologies are aiming to meet renewable fuel standards, mitigate greenhouse gas emissions, and provide viable alternatives to fossil fuels. Direct conversion of solar energy into fungible liquid fuel is ...