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Now showing items 21-30 of 176
An Integrated Strategy for Analyzing the Unique Developmental Programs of Different Myoblast Subtypes
(Public Library of Science, 2006)
An important but largely unmet challenge in understanding the mechanisms that govern the formation of specific organs is to decipher the complex and dynamic genetic programs exhibited by the diversity of cell types within ...
Proteome-Wide Systems Analysis of a Cellulosic Biofuel-Producing Microbe
(Nature Publishing Group, 2011)
Fermentation of plant biomass by microbes like Clostridium phytofermentans recycles carbon globally and can make biofuels from inedible feedstocks. We analyzed C. phytofermentans fermenting cellulosic substrates by integrating ...
Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers
(Oxford University Press, 2012)
DNA built from modular repeats presents a challenge for gene synthesis. We present a solid surface-based sequential ligation approach, which we refer to as iterative capped assembly (ICA), that adds DNA repeat monomers ...
Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases
(Public Library of Science, 2012)
Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination. Previous studies have suggested that endogenous ...
Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection
(Oxford University Press, 2012)
Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is the directed integration of chemically synthesized single-stranded ...
Next Generation Gene Synthesis by targeted retrieval of bead-immobilized, sequence verified DNA clones from a high throughput pyrosequencing device
(2012)
The setup of synthetic biological systems involving millions of bases is still limited by the required high quality of synthetic DNA. Important drivers to further open up the field are the accuracy and scale of chemical ...
Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases
(Public Library of Science, 2012)
Recent advances allow multiplexed genome engineering in E. coli, employing easily designed oligonucleotides to edit multiple loci simultaneously. A similar technology in human cells would greatly expedite functional genomics, ...
Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing
(Public Library of Science, 2012)
High-throughput recording of signals embedded within inaccessible micro-environments is a technological challenge. The ideal recording device would be a nanoscale machine capable of quantitatively transducing a wide range ...
Reading and writing omes
(Nature Publishing Group, 2013)
Genome-Scale Promoter Engineering by Coselection MAGE
(Nature Publishing Group, 2012)
Multiplex Automated Genome Engineering (MAGE) employs short oligonucleotides to scarlessly modify genomes. However, insertions of >10 bases are still inefficient, but can be improved substantially by selection of highly ...