Browsing HMS Scholarly Articles by Author "Hyberts, Sven"
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Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression
Linser, Rasmus; Gelev, Vladimir; Hagn, Franz; Arthanari, Haribabu; Hyberts, Sven G.; Wagner, Gerhard (American Chemical Society, 2014)Structural characterization of membrane proteins and other large proteins with NMR relies increasingly on perdeuteration combined with incorporation of specifically protonated amino acid moieties, such as methyl groups of ... -
Solid-State NMR Structure Determination from Diagonal-Compensated, Sparsely Nonuniform-Sampled 4D Proton–Proton Restraints
Linser, Rasmus; Bardiaux, Benjamin; Andreas, Loren B.; Hyberts, Sven G.; Morris, Vanessa K.; Pintacuda, Guido; Sunde, Margaret; Kwan, Ann H.; Wagner, Gerhard (American Chemical Society, 2014)We report acquisition of diagonal-compensated protein structural restraints from four-dimensional solid-state NMR spectra on extensively deuterated and 1H back-exchanged proteins. To achieve this, we use homonuclear 1H–1H ...