Browsing HMS Scholarly Articles by Author "Briggs, Adrian"

Now showing items 1-3 of 3

    • Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers 

      Briggs, Adrian; Rios, Xavier; Chari, Rajagopal; Yang, Luhan; Zhang, Feng; Mali, Prashant; Church, George McDonald (Oxford University Press, 2012)
      DNA built from modular repeats presents a challenge for gene synthesis. We present a solid surface-based sequential ligation approach, which we refer to as iterative capped assembly (ICA), that adds DNA repeat monomers ...

    • Optimization of scarless human stem cell genome editing 

      Yang, Luhan; Guell, Marc; Byrne, Susan; Yang, Joyce L.; De Los Angeles, Alejandro; Mali, Prashant; Aach, John; Kim-Kiselak, Caroline; Briggs, Adrian W; Rios, Xavier; Huang, Po-Yi; Daley, George; Church, George (Oxford University Press, 2013)
      Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and clinical purposes. The development of programmable sequence-specific ...

    • Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases 

      Rios, Xavier; Briggs, Adrian; Christodoulou, Danos C.; Gorham, Josh McClean; Seidman, Jonathan G.; Church, George McDonald (Public Library of Science, 2012)
      Recent advances allow multiplexed genome engineering in E. coli, employing easily designed oligonucleotides to edit multiple loci simultaneously. A similar technology in human cells would greatly expedite functional genomics, ...