Browsing HMS Scholarly Articles by Title

Now showing items 16175-16194 of 17922

    • Synthetic Biology Projects in Vitro 

      Forster, Anthony C.; Church, George (Cold Spring Harbor Laboratory, 2007-01)
      Advances in the in vitro synthesis and evolution of DNA, RNA, and polypeptides are accelerating the construction of biopolymers, pathways, and organisms with novel functions. Known functions are being integrated and debugged ...

    • Synthetic Biology: Advancing Biological Frontiers by Building Synthetic Systems 

      Chen, Yvonne Yu-Hsuan; Galloway, Kate E; Smolke, Christina D (BioMed Central, 2012)
      Advances in synthetic biology are contributing to diverse research areas, from basic biology to biomanufacturing and disease therapy. We discuss the theoretical foundation, applications, and potential of this emerging field.

    • Synthetic biosensors for precise gene control and real-time monitoring of metabolites 

      Rogers, Jameson K.; Guzman, Christopher D.; Taylor, Noah D.; Raman, Srivatsan; Anderson, Kelley; Church, George M. (Oxford University Press, 2015)
      Characterization and standardization of inducible transcriptional regulators has transformed how scientists approach biology by allowing precise and tunable control of gene expression. Despite their utility, only a handful ...

    • Synthetic design of strong promoters 

      Schlabach, Michael R.; Hu, Jimmy K.; Li, Mamie; Elledge, Stephen J. (National Academy of Sciences, 2010)
      We have taken a synthetic biology approach to the generation and screening of transcription factor binding sites for activity in human cells. All possible 10-mer DNA sequences were printed on microarrays as 100-mers ...

    • Synthetic DNA spike-ins (SDSIs) enable sample tracking and detection of inter-sample contamination in SARS-CoV-2 sequencing workflows 

      Lagerborg, Kim A; Normandin, Erica; Bauer, Matthew; Adams, Gordon; Figueroa, Katherine; Loreth, Christine; Gladden-Young, Adrianne; Shaw, Bennett; Pearlman, Leah; Berenzy, Daniel; Dewey, Hannah; Kales, Susan; Dobbins, Sabrina; Seiguer Shenoy, Erica; Hooper, David; Pierce, Virginia; Zachary, Kimon; Park, Daniel; Macinnis, Bronwyn; Tewhey, Ryan; Lemieux, Jacob; Sabeti, Pardis; Reilly, Steven; Siddle, Katherine (Springer Science and Business Media LLC, 2021-12-14)
      The global spread and continued evolution of SARS-CoV-2 has driven an unprecedented surge in viral genomic surveillance. Amplicon-based sequencing methods provide a sensitive, low-cost and rapid approach but suffer a high ...

    • A Synthetic Form of Frizzled 8-Associated Antiproliferative Factor Enhances p53 Stability through USP2a and MDM2 

      Kim, Jayoung; Keay, Susan K.; You, Sungyong; Loda, Massimo; Freeman, Michael R. (Public Library of Science, 2012)
      Frizzled 8-associated Antiproliferative Factor (APF) is a sialoglycopeptide urinary biomarker of interstitial cystitis/painful bladder syndrome (IC/PBS), a chronic condition of unknown etiology with variable symptoms that ...

    • Synthetic Gene Networks That Count 

      Friedland, Ari E.; Lu, Timothy K.; Wang, Xiao; Shi, David; Church, George; Collins, James (American Association for the Advancement of Science, 2009-05-29)
      Synthetic gene networks can be constructed to emulate digital circuits and devices, giving one the ability to program and design cells with some of the principles of modern computing, such as counting. A cellular counter ...

    • A synthetic lethal approach for compound and target identification in Staphylococcus aureus 

      Pasquina, Lincoln; Maria, John P. Santa; Wood, B. McKay; Moussa, Samir H.; Matano, Leigh; Santiago, Marina; Martin, Sara E. S.; Lee, Wonsik; Meredith, Timothy C.; Walker, Suzanne (2015)
      The majority of bacterial proteins are dispensable for growth in the laboratory, but nevertheless play important physiological roles. There are no systematic approaches to identify cell-permeable small molecule inhibitors ...

    • Synthetic lethality between CCNE1 amplification and loss of BRCA1 

      Etemadmoghadam, Dariush; Weir, Barbara A.; Au-Yeung, George; Alsop, Kathryn; Mitchell, Gillian; George, Joshy; Davis, Sally; D’Andrea, Alan D.; Simpson, Kaylene; Hahn, William C.; Bowtell, David L. (National Academy of Sciences, 2013)
      High-grade serous ovarian cancers (HGSCs) are characterized by a high frequency of TP53 mutations, BRCA1/2 inactivation, homologous recombination dysfunction, and widespread copy number changes. Cyclin E1 (CCNE1) gene ...

    • Synthetic photosynthetic consortia define interactions leading to robustness and photoproduction 

      Hays, Stephanie G.; Yan, Leo L. W.; Silver, Pamela A.; Ducat, Daniel C. (BioMed Central, 2017)
      Background: Microbial consortia composed of autotrophic and heterotrophic species abound in nature, yet examples of synthetic communities with mixed metabolism are limited in the laboratory. We previously engineered a model ...

    • The Synthetic Tie2 Agonist Peptide Vasculotide Protects Against Vascular Leakage and Reduces Mortality in Murine Abdominal Sepsis 

      Kümpers, Philipp; Gueler, Faikah; David, Sascha; Van Slyke, Paul; Dumont, Daniel J; Park, Joon-Keun; Bockmeyer, Clemens L; Pavenstädt, Hermann; Haller, Hermann; Shushakova, Nelli; Parikh, Samir Mukund (BioMed Central, 2011)
      Introduction: Angiopoietin-1 (Angpt1), the natural agonist ligand for the endothelial Tie2 receptor, is a non-redundant endothelial survival and vascular stabilization factor that reduces endothelial permeability and ...

    • Syntrophic exchange in synthetic microbial communities 

      Mee, MT; Collins, JJ; Wang, HH; Church, George (Proceedings of the National Academy of Sciences, 2014-04-28)
      Metabolic crossfeeding is an important process that can broadly shape microbial communities. However, little is known about specific crossfeeding principles that drive the formation and maintenance of individuals within a ...

    • Syphilis Predicts HIV Incidence Among Men and Transgender Women Who Have Sex With Men in a Preexposure Prophylaxis Trial 

      Solomon, Marc M.; Mayer, Kenneth H.; Glidden, David V.; Liu, Albert Y.; McMahan, Vanessa M.; Guanira, Juan V.; Chariyalertsak, Suwat; Fernandez, Telmo; Grant, Robert M.; Bekker, Linda-Gail; Buchbinder, Susan; Casapia, Martin; Guanira, Juan; Kallas, Esper; Lama, Javier; Mayer, Kenneth; Montoya, Orlando; Schechter, Mauro; Veloso, Valdiléa (Oxford University Press, 2014)
      Background. Syphilis infection may potentiate transmission of human immunodeficiency virus (HIV). We sought to determine the extent to which HIV acquisition was associated with syphilis infection within an HIV preexposure ...

    • Syrian refugees, between rocky crisis in Syria and hard inaccessibility to healthcare services in Lebanon and Jordan 

      El-Khatib, Ziad; Scales, David; Vearey, Jo; Forsberg, Birger C (BioMed Central, 2013)
      Around 3% of the world’s population (n = 214 million people) has crossed international borders for various reasons. Since March 2011, Syria has been going through state of political crisis and instability resulting in an ...

    • A System for Household Enumeration and Re-identification in Densely Populated Slums to Facilitate Community Research, Education, and Advocacy 

      Thomson, Dana R.; Shitole, Shrutika; Shitole, Tejal; Sawant, Kiran; Subbaraman, Ramnath; Bloom, David E.; Patil-Deshmukh, Anita (Public Library of Science, 2014)
      Background: We devised and implemented an innovative Location-Based Household Coding System (LBHCS) appropriate to a densely populated informal settlement in Mumbai, India. Methods and Findings: LBHCS codes were designed ...

    • A systematic analysis of a broadly neutralizing antibody AR3C epitopes on Hepatitis C virus E2 envelope glycoprotein and their cross-reactivity 

      Sun, Jing; Brusic, Vladimir (BioMed Central, 2015)
      Background: Hepatitis C virus (HCV) belongs to Flaviviridae family of viruses. HCV represents a major challenge to public health since its estimated global prevalence is 2.8% of the world's human population. The design and ...

    • Systematic analysis of BRAFV600E melanomas reveals a role for JNK/c-Jun pathway in adaptive resistance to drug-induced apoptosis 

      Fallahi-Sichani, Mohammad; Moerke, Nathan J; Niepel, Mario; Zhang, Tinghu; Gray, Nathanael S; Sorger, Peter K (BlackWell Publishing Ltd, 2015)
      Drugs that inhibit RAF/MEK signaling, such as vemurafenib, elicit profound but often temporary anti-tumor responses in patients with BRAFV600E melanoma. Adaptive responses to RAF/MEK inhibition occur on a timescale of hours ...

    • A Systematic Analysis of Factors Localized to Damaged Chromatin Reveals PARP-Dependent Recruitment of Transcription Factors 

      Izhar, Lior; Adamson, Britt; Ciccia, Alberto; Lewis, Jedd; Pontano-Vaites, Laura; Leng, Yumei; Westbrook, Thomas F.; Elledge, Stephen; Liang, Anthony; Harper, Jeffrey (Elsevier BV, 2015-06)
      Localization to sites of DNA damage is a hallmark of DNA damage response (DDR) proteins. To identify DDR factors, we screened epitope-tagged proteins for localization to sites of chromatin damaged by UV laser microirradiation ...

    • Systematic Analysis of Intracellular Trafficking Motifs Located within the Cytoplasmic Domain of Simian Immunodeficiency Virus Glycoprotein gp41 

      Postler, Thomas S.; Bixby, Jacqueline G.; Desrosiers, Ronald C.; Yuste, Eloísa (Public Library of Science, 2014)
      Previous studies have shown that truncation of the cytoplasmic-domain sequences of the simian immunodeficiency virus (SIV) envelope glycoprotein (Env) just prior to a potential intracellular-trafficking signal of the ...

    • Systematic Analysis of Sex-Linked Molecular Alterations and Therapies in Cancer 

      Ma, Jonathan; Malladi, Sadhika; Beck, Andrew H (Nature Publishing Group, 2016)
      Though patient sex influences response to cancer treatments, little is known of the molecular causes, and cancer therapies are generally given irrespective of patient sex. We assessed transcriptomic differences in tumors ...