Browsing HMS Scholarly Articles by Title
Now showing items 15353-15372 of 17922
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Single-cell ChIP-seq reveals cell subpopulations defined by chromatin state
(2015)Chromatin profiling provides a versatile means to investigate functional genomic elements and their regulation. However, current methods yield ensemble profiles that are insensitive to cell-to-cell variation. Here we combine ... -
Single-cell delineation of lineage and genetic identity in the mouse brain
(Springer Science and Business Media LLC, 2021-12-15)<jats:title>Abstract</jats:title><jats:p>During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of ... -
Single-cell delineation of lineage and genetic identity in the mouse brain
(Springer Science and Business Media LLC, 2021-12-15)During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of postmitotic neurons and glia. The link between ... -
Single-cell eQTL models reveal dynamic T cell state dependence of disease loci
(Springer Science and Business Media LLC, 2022-05-11)Non-coding genetic variants may cause disease by modulating gene expression. However, identifying these expression quantitative trait loci (eQTLs) is complicated by gene-regulation differences across fluid functional cell ... -
Single-Cell Lineage Tracing Unveils a Role for TCF15 in Haematopoiesis
(Springer Science and Business Media LLC, 2020-07-15)Bone marrow transplantation therapy relies on the life-long regenerative capacity of haematopoietic stem cells (HSCs). HSCs present a complex variety of regenerative behaviours at the clonal level, but the mechanisms ... -
Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics
(Springer Science and Business Media LLC, 2021-03)ACE2 and accessory proteases (TMPRSS2, CTSL) are needed for SARS-CoV-2 cellular entry, and their expression may shed light on viral tropism and impact across the body. We assess the cell type-specific expression of ACE2, ... -
Single-cell pharmacokinetic imaging reveals a therapeutic strategy to overcome drug resistance to the microtubule inhibitor eribulin
(American Association for the Advancement of Science, 2014)Eribulin mesylate was developed as a potent microtubule-targeting cytotoxic agent to treat taxane-resistant cancers, but recent clinical trials have shown that it eventually fails in many patient subpopulations for unclear ... -
Single-Cell RNA Sequencing Identifies Extracellular Matrix Gene Expression by Pancreatic Circulating Tumor Cells
(2014)SUMMARY Circulating tumor cells (CTCs) are shed from primary tumors into the bloodstream, mediating the hematogenous spread of cancer to distant organs. To define their composition, we compared genome-wide expression ... -
Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma
(American Association for the Advancement of Science (AAAS), 2014)Human cancers are complex ecosystems composed of cells with distinct phenotypes, genotypes and epigenetic states, but current models do not adequately reflect tumor composition in patients. We used single cell RNA-seq to ... -
Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity
(Elsevier BV, 2017)Chen et al. perform single-cell RNA sequencing analysis of the adult mouse hypothalamus to probe the rich cell diversity of this complex brain region. They also identify neuronal subtypespecific transcriptional responses ... -
Single-Cell Transcript Profiles Reveal Multilineage Priming in Early Progenitors Derived from Lgr5 + Intestinal Stem Cells
(Elsevier BV, 2016-08)Lgr5+ intestinal stem cells (ISC) drive epithelial self-renewal, and their immediate progeny – intestinal bipotential progenitors – produce absorptive and secretory lineages via lateral inhibition. To define features of ... -
Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish
(The Rockefeller University Press, 2016)Hematopoiesis culminates in the production of functionally heterogeneous blood cell types. In zebrafish, the lack of cell surface antibodies has compelled researchers to use fluorescent transgenic reporter lines to label ... -
Single-Cell Transcriptomic Analysis of Alzheimer’s Disease
(Springer Science and Business Media LLC, 2019-05-01)Alzheimer’s disease (AD) is a pervasive neurodegenerative disorder whose molecular and cellular complexity remains poorly understood. Here, we profiled and analyzed 80,660 single-nucleus transcriptomes from prefrontal ... -
Single-cell transcriptomic profiling of the aging mouse brain
(Springer Science and Business Media LLC, 2019-09-24)The mammalian brain is complex, with multiple cell types performing a variety of diverse functions, but exactly how each cell type is affected in aging remains largely unknown. Here we performed a single-cell transcriptomic ... -
Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells
(2013)Recent molecular studies have revealed that, even when derived from a seemingly homogenous population, individual cells can exhibit substantial differences in gene expression, protein levels, and phenotypic output1–5, with ... -
Single-Cell, Genome-wide Sequencing Identifies Clonal Somatic Copy-Number Variation in the Human Brain
(2014)SUMMARY De novo copy-number variants (CNVs) can cause neuropsychiatric disease, but the degree to which they occur somatically, and during development, is unknown. Single-cell whole-genome sequencing (WGS) in >200 single ... -
Single-molecule studies of polymerase dynamics and stoichiometry at the bacteriophage T7 replication machinery
(National Academy of Sciences, 2014)Replication of DNA plays a central role in transmitting hereditary information from cell to cell. To achieve reliable DNA replication, multiple proteins form a stable complex, known as the replisome, enabling them to act ... -
Single-Molecule Study on Histone-Like Nucleoid-Structuring Protein (H-NS) Paralogue in Pseudomonas aeruginosa: MvaU Bears DNA Organization Mode Similarities to MvaT
(Public Library of Science, 2014)Pseudomonas aeruginosa contains two distinct members of H-NS family of nucleoid-structuring proteins: MvaT and MvaU. Together, these proteins bind to the same regions of the chromosome and function coordinately in the ... -
Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes
(Nature Pub. Group, 2015)Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the in situ visualization ... -
Single-neuron correlates of subjective vision in the human medial temporal lobe
(Proceedings of the National Academy of Sciences, 2002)Visual information from the environment is transformed into perceptual sensations through several stages of neuronal processing. Flash suppression constitutes a striking example in which the same retinal input can give ...