Browsing HMS Scholarly Articles by Title
Now showing items 95-114 of 17921
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A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
(National Academy of Sciences, 2005)The advent of RNA interference has led to the ability to interfere with gene expression and greatly expanded our ability to perform genetic screens in mammalian cells. The expression of short hairpin RNA (shRNA) from ... -
A ligand-binding pocket in the dengue virus envelope glycoprotein
(National Academy of Sciences, 2003)Dengue virus is an emerging global health threat. Its major envelope glycoprotein, E, mediates viral attachment and entry by membrane fusion. A crystal structure of the soluble ectodomain of E from dengue virus type 2 ... -
A Light-activated Theranostic Nanoagent for Targeted Macrophage Ablation in Inflammatory Atherosclerosis
(Wiley, 2010)The synthesis and utility of a multimodal theranostic nanoagent based upon magnetofluorescent nanoparticles for the treatment of inflammatory atherosclerosis is described. These particles are modified with near-infrared ... -
A magneto-DNA nanoparticle system for rapid detection and phenotyping of bacteria
(Nature Research, 2013)So far, although various diagnostic approaches for pathogen detection have been proposed, most are too expensive, lengthy or limited in specificity for clinical use. Nanoparticle systems with unique material properties, ... -
A Metasystem of Framework Model Organisms to Study Emergence of New Host-Microbe Adaptations
(Public Library of Science, 2008)An unintended consequence of global industrialization and associated societal rearrangements is new interactions of microbes and potential hosts (especially mammals and plants), providing an opportunity for the rapid ... -
A Mitotic Cascade of Nima Family Kinases Nercc1/nek9 Activates the Nek6 and Nek7 Kinases
(American Society for Biochemistry and Molecular Biology, 2003)The Nek family of protein kinases in humans is composed of 11 members that share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent ... -
A modulatory role for facial expressions in prosopagnosia
(National Academy of Sciences, 2003)Brain-damaged patients experience difficulties in recognizing a face (prosopagnosics), but they can still recognize its expression. The dissociation between these two face-related skills has served as a keystone of models ... -
A Motif Co-Occurrence Approach for Genome-Wide Prediction of Transcription-Factor-Binding Sites in Escherichia coli
(Cold Spring Harbor Laboratory Press, 2004)Various computational approaches have been developed for predicting cis-regulatory DNA elements in prokaryotic genomes. We describe a novel method for predicting transcription-factor-binding sites in Escherichia coli. Our ... -
A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole
(Cold Spring Harbor Laboratory Press, 2012)The cell poles constitute key subcellular domains that are often critical for motility, chemotaxis, and chromosome segregation in rod-shaped bacteria. However, in nearly all rods, the processes that underlie the formation, ... -
A Multifunctional Single-Attachment-Point Reagent for Controlled Protein Biotinylation
(American Chemical Society, 2009)The biotin/avidin system is one of the most widely used affinity detection and affinity capture systems in biology. However, the determination of the exact number of biotin tags attached onto a substrate is complicated by ... -
A Multimodal Nanoparticle for Preoperative Magnetic Resonance Imaging and Intraoperative Optical Brain Tumor Delineation
(American Association for Cancer Research, 2003)The determination of brain tumor margins both during the presurgical planning phase and during surgical resection has long been a challenging task in the therapy of brain tumor patients. Using a model of gliosarcoma, with ... -
A Mutation Deleting Sequences Encoding the Amino Terminus of Human Cytomegalovirus UL84 Impairs Interaction with UL44 and Capsid Localization
(American Society for Microbiology, 2012)Protein-protein interactions are required for many biological functions. Previous work has demonstrated an interaction between the human cytomegalovirus DNA polymerase subunit UL44 and the viral replication factor UL84. ... -
A Mutation in the Gene-encoding Bacteriophage T7 DNA Polymerase That Renders the Phage Temperature-sensitive
(American Society for Biochemistry and Molecular Biology, 2001)Gene 5 of bacteriophage T7 encodes a DNA polymerase essential for phage replication. A single point mutation in gene 5 confers temperature sensitivity for phage growth. The mutation results in an alanine to valine substitution ... -
A New Class of Allosteric HIV-1 Integrase Inhibitors Identified by Crystallographic Fragment Screening of the Catalytic Core Domain
(American Society for Biochemistry and Molecular Biology, 2016)HIV-1 integrase (IN) is essential for virus replication and represents an important multifunctional therapeutic target. Recently discovered quinoline-based allosteric IN inhibitors (ALLINIs) potently impair HIV-1 replication ... -
A Non-Canonical Function of Zebrafish Telomerase Reverse Transcriptase Is Required for Developmental Hematopoiesis
(Public Library of Science, 2008)Although it is clear that telomerase expression is crucial for the maintenance of telomere homeostasis, there is increasing evidence that the TERT protein can have physiological roles that are independent of this central ... -
A novel chemical screening strategy in zebrafish identifies common pathways in embryogenesis and rhabdomyosarcoma development
(Company of Biologists, 2013)The zebrafish is a powerful genetic model that has only recently been used to dissect developmental pathways involved in oncogenesis. We hypothesized that operative pathways during embryogenesis would also be used for ... -
A novel fluorescent probe for the detection of myeloperoxidase activity in atherosclerosis-associated macrophages
(Elsevier (Cell Press), 2007)The myeloperoxidase (MPO)-derived oxidant hypochlorous acid (HOCI/OCI-) is implicated in the pathogenesis of atherosclerosis and other inflammatory states. We have synthesized an imaging probe, sulfonaphthoamino-phenyl ... -
A Novel Method for Imaging Apoptosis Using a Caspase-1 Near-Infrared Fluorescent Probe
(Elsevier, 2004)Here we describe a novel method for imaging apoptosis in cells using a near-infrared fluorescent (NRF) probe selective for caspase-1 (interleukin 1beta-converting enzyme, ICE). This biocompatible, optically quenched ICE-NIRF ... -
A Novel Molecule Integrating Therapeutic and Diagnostic Activities Reveals Multiple Aspects of Stem Cell-Based Therapy
(AlphaMed Press, 2010)Stem cells are promising therapeutic delivery vehicles; however pre-clinical and clinical applications of stem cell-based therapy would benefit significantly from the ability to simultaneously determine therapeutic efficacy ... -
A PHD Finger Motif in the C Terminus of RAG2 Modulates Recombination Activity
(American Society for Biochemistry and Molecular Biology, 2005)The RAG1 and RAG2 proteins catalyze V( D) J recombination and are essential for generation of the diverse repertoire of antigen receptor genes and effective immune responses. RAG2 is composed of a "core" domain that is ...