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dc.contributor.authorKim, Joanne SM
dc.contributor.authorLilley, Brendan Neil
dc.contributor.authorZhang, Chao
dc.contributor.authorShokat, Kevan M
dc.contributor.authorSanes, Joshua R.
dc.contributor.authorZhen, Mei
dc.date.accessioned2010-10-01T18:34:20Z
dc.date.issued2008
dc.identifier.citationKim, Joanne SM, Brendan N. Lilley, Chao Zhang, Kevan M. Shokat, Joshua R. Sanes, and Mei Zhen. 2008. A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation. Neural Development 3:23.en_US
dc.identifier.issn1749-8104en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4455249
dc.description.abstractBackground: Neurons assemble into a functional network through a sequence of developmental processes including neuronal polarization and synapse formation. In Caenorhabditis elegans, the serine/threonine SAD-1 kinase is essential for proper neuronal polarity and synaptic organization. To determine if SAD-1 activity regulates the establishment or maintenance of these neuronal structures, we examined its temporal requirements using a chemical-genetic method that allows for selective and reversible inactivation of its kinase activity in vivo. Results: We generated a PP1 analog-sensitive variant of SAD-1. Through temporal inhibition of SAD-1 kinase activity we show that its activity is required for the establishment of both neuronal polarity and synaptic organization. However, while SAD-1 activity is needed strictly when neurons are polarizing, the temporal requirement for SAD-1 is less stringent in synaptic organization, which can also be re-established during maintenance. Conclusion: This study reports the first temporal analysis of a neural kinase activity using the chemical-genetic system. It reveals that neuronal polarity and synaptic organization have distinct temporal requirements for SAD-1.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1749-8104-3-23en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2564922/pdf/en_US
dash.licenseOAP
dc.titleA Chemical-Genetic Strategy Reveals Distinct Temporal Requirements for SAD-1 Kinase in Neuronal Polarization and Synapse Formationen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNeural Developmenten_US
dash.depositing.authorSanes, Joshua R.
dc.date.available2010-10-01T18:34:20Z
dc.identifier.doi10.1186/1749-8104-3-23*
dash.contributor.affiliatedLilley, Brendan Neil
dash.contributor.affiliatedSanes, Joshua


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