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dc.contributor.authorAnderson, William J.
dc.contributor.authorZhou, Qiao
dc.contributor.authorAlcalde, Victor
dc.contributor.authorKaneko, Osamu F
dc.contributor.authorBlank, Leah J
dc.contributor.authorSherwood, Richard Irving
dc.contributor.authorGuseh, James Sawalla
dc.contributor.authorRajagopal, Jayaraj
dc.contributor.authorMelton, Douglas A.
dc.date.accessioned2010-10-06T17:55:32Z
dc.date.issued2008
dc.identifier.citationAnderson, William J., Qiao Zhou, Victor Alcalde, Osamu F. Kaneko, Leah J. Blank, Richard I. Sherwood, J. Sawalla Guseh, Jayaraj Rajagopal, and Douglas A. Melton. 2008. Genetic targeting of the endoderm with claudin-6. Developmental Dynamics 237(2): 504-512.en_US
dc.identifier.issn1058-8388en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4459990
dc.description.abstractA full description of the ontogeny of the β cell would guide efforts to generate β cells from embryonic stem cells (ESCs). The first step requires an understanding of definitive endoderm: the genes and signals responsible for its specification, proliferation, and patterning. This report describes a global marker of definitive endoderm, Claudin-6 (Cldn6). We report its expression in early development with particular attention to definitive endoderm derivatives. To create a genetic system to drive gene expression throughout the definitive endoderm with both spatial and temporal control, we target the endogenous locus with an inducible Cre recombinase (Cre-ERT2) cassette. Cldn6 null mice are viable and fertile with no obvious phenotypic abnormalities. We also report a lineage analysis of the fate of Cldn6-expressing embryonic cells, which is relevant to the development of the pancreas, lung, and liver.en_US
dc.description.sponsorshipStem Cell and Regenerative Biologyen_US
dc.language.isoen_USen_US
dc.publisherWiley-Blackwellen_US
dc.relation.isversionofdoi:10.1002/dvdy.21437en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665265/pdf/en_US
dash.licenseLAA
dc.subjectendodermen_US
dc.subjectCldn6en_US
dc.subjectCre-ERen_US
dc.subjectembryonic stem cellsen_US
dc.subjectorganogenesisen_US
dc.subjectgene targetingen_US
dc.subjectlineage analysisen_US
dc.titleGenetic Targeting of the Endoderm with Claudin-6CreERen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalDevelopmental Dynamicsen_US
dash.depositing.authorMelton, Douglas A.
dc.date.available2010-10-06T17:55:32Z
dc.identifier.doi10.1002/dvdy.21437*
dash.contributor.affiliatedAnderson, William
dash.contributor.affiliatedGuseh, James
dash.contributor.affiliatedSherwood, Richard
dash.contributor.affiliatedRajagopal, Jayaraj
dash.contributor.affiliatedMelton, Douglas
dash.contributor.affiliatedZhou, Qiao


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