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dc.contributor.authorMao, Junhao
dc.contributor.authorBarrow, Jeffery
dc.contributor.authorMcMahon, Jill Ann
dc.contributor.authorVaughan, Joe
dc.contributor.authorMcMahon, Andrew P.
dc.date.accessioned2010-10-06T19:23:20Z
dc.date.issued2005
dc.identifier.citationMao, Junhao, Jeffery Barrow, Jill McMahon, Joe Vaughan, and Andrew P. McMahon. 2005. An ES cell system for rapid, spatial and temporal analysis of gene function and . Nucleic Acids Research 33(18): e155.en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4460784
dc.description.abstractWe describe a versatile genetic system for rapid analysis of mammalian gene function. In this, loss of reporter activity in a novel embryonic stem (ES) cell line enables rapid identification of targeting to the ubiquitously expressed Rosa26 locus. Subsequent regulation of gene activity is governed by a dual regulatory strategy utilizing two drugs, Tamoxifen and Doxycycline. To illustrate this approach, a dominant allele of Smoothened was introduced into this cell line, enabling regulated activation of Hedgehog signaling. By coupling Cre-loxP dependent activation with tetracycline dependent transcription in a single allele, we established a conditional method to control Smoothened activity and neural progenitor specification in differentiating ES cells in vitro and in chimeric embryos in vivo When crossed to an appropriate Cre driver strain, gene activity can also be temporally regulated within a specific cell lineage. This platform will facilitate rapid analysis of gene function in the mouse.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.description.sponsorshipStem Cell and Regenerative Biologyen_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isversionofdoi:10.1093/nar/gni146en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1253836/pdf/en_US
dash.licenseLAA
dc.titleAn ES Cell System for Rapid, Spatial and Temporal Analysis of Gene Function in vitro and in vivoen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNucleic Acids Researchen_US
dash.depositing.authorMcMahon, Andrew P.
dc.date.available2010-10-06T19:23:20Z
dc.identifier.doi10.1093/nar/gni146*
dash.authorsorderedfalse
dash.contributor.affiliatedMcMahon, Andrew P.
dash.contributor.affiliatedMcMahon, Jill Ann


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