Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network
View/ Open
Author
Ejsing, Christer S.
Surma, Michal A.
Kaiser, Hermann-Josef
Gerl, Mathias J.
Sampaio, Julio L.
de Robillard, Quentin
Ferguson, Charles
Shevchenko, Andrej
Simons, Kai
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1083/jcb.200901145Metadata
Show full item recordCitation
Klemm, Robin W., Christer S. Ejsing, Michal A. Surma, Hermann-Josef Kaiser, Mathias J. Gerl, Julio L. Sampaio, Quentin de Robillard, et al. 2009. Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network. The Journal of Cell Biology 185(4): 601-612.Abstract
The trans-Golgi network (TGN) is the major sorting station in the secretory pathway of all eukaryotic cells. How the TGN sorts proteins and lipids to generate the enrichment of sphingolipids and sterols at the plasma membrane is poorly understood. To address this fundamental question in membrane trafficking, we devised an immunoisolation procedure for specific recovery of post-Golgi secretory vesicles transporting a transmembrane raft protein from the TGN to the cell surface in the yeast Saccharomyces cerevisiae. Using a novel quantitative shotgun lipidomics approach, we could demonstrate that TGN sorting selectively enriched ergosterol and sphingolipid species in the immunoisolated secretory vesicles. This finding, for the first time, indicates that the TGN exhibits the capacity to sort membrane lipids. Furthermore, the observation that the immunoisolated vesicles exhibited a higher membrane order than the late Golgi membrane, as measured by C-Laurdan spectrophotometry, strongly suggests that lipid rafts play a role in the TGN-sorting machinery.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711577/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:4513029
Collections
- FAS Scholarly Articles [18292]
Contact administrator regarding this item (to report mistakes or request changes)