Live Imaging of Cysteine-Cathepsin Activity Reveals Dynamics of Focal Inflammation, Angiogenesis, and Polyp Growth

DSpace/Manakin Repository

Live Imaging of Cysteine-Cathepsin Activity Reveals Dynamics of Focal Inflammation, Angiogenesis, and Polyp Growth

Show simple item record

dc.contributor.author Gounaris, Elias
dc.contributor.author Tung, Ching H.
dc.contributor.author Restaino, Clifford
dc.contributor.author Joyce, Johanna A.
dc.contributor.author Plough, Hidde L.
dc.contributor.author Barrett, Terrence A.
dc.contributor.author Khazaie, Khashayarsha
dc.contributor.author Maehr, Rene
dc.contributor.author Kohler, Rainer H.
dc.contributor.author Weissleder, Ralph
dc.date.accessioned 2010-11-01T17:00:15Z
dc.date.issued 2008
dc.identifier.citation Gounaris, Elias, Ching H. Tung, Clifford Restaino, René Maehr, Rainer Kohler, Johanna A. Joyce, Hidde L. Plough, Terrence A. Barrett, Ralph Weissleder, and Khashayarsha Khazaie. 2008. Live imaging of cysteine-cathepsin activity reveals dynamics of focal inflammation, angiogenesis, and polyp growth. PLoS ONE 3:e2916. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4515009
dc.description.abstract It has been estimated that up to 30% of detectable polyps in patients regress spontaneously. One major challenge in the evaluation of effective therapy of cancer is the readout for tumor regression and favorable biological response to therapy. Inducible near infra-red (NIR) fluorescent probes were utilized to visualize intestinal polyps of mice hemizygous for a novel truncation of the Adenomatous Polyposis coli (APC) gene. Laser Scanning Confocal Microscopy in live mice allowed visualization of cathepsin activity in richly vascularized benign dysplastic lesions. Using biotinylated suicide inhibitors we quantified increased activities of the Cathepsin B & Z in the polyps. More than ¾ of the probe signal was localized in CD11b+Gr1+ myeloid derived suppressor cells (MDSC) and CD11b+F4/80+ macrophages infiltrating the lesions. Polyposis was attenuated through genetic ablation of cathepsin B, and suppressed by neutralization of TNFα in mice. In both cases, diminished probe signal was accounted for by loss of MDSC. Thus, in vivo NIR imaging of focal cathepsin activity reveals inflammatory reactions etiologically linked with cancer progression and is a suitable approach for monitoring response to therapy. en_US
dc.description.sponsorship Stem Cell and Regenerative Biology en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pone.0002916 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2488397/pdf/ en_US
dash.license LAA
dc.subject radiology and medical imaging en_US
dc.subject immunology en_US
dc.subject innate immunity en_US
dc.subject gastroenterology and hepatology en_US
dc.subject gastrointestinal cancers en_US
dc.title Live Imaging of Cysteine-Cathepsin Activity Reveals Dynamics of Focal Inflammation, Angiogenesis, and Polyp Growth en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Maehr, Rene
dc.date.available 2010-11-01T17:00:15Z

Files in this item

Files Size Format View
2488397.pdf 566.8Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

 
 

Search DASH


Advanced Search
 
 

Submitters