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dc.contributor.authorDanley, Patrick D
dc.contributor.authorMullen, Sean P
dc.contributor.authorLiu, Fenglong
dc.contributor.authorNene, Vishvanath
dc.contributor.authorQuackenbush, John
dc.contributor.authorShaw, Kerry L
dc.date.accessioned2010-11-08T16:39:11Z
dc.date.issued2007
dc.identifier.citationDanley, Patrick D., Sean P. Mullen, Fenglong Liu, Vishvanath Nene, John Quackenbush, and Kerry L. Shaw. 2007. A cricket gene index: a genomic resource for studying neurobiology, speciation, and molecular evolution. BMC Genomics 8:109.en_US
dc.identifier.issn1471-2164en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4551296
dc.description.abstractBackground: As the developmental costs of genomic tools decline, genomic approaches to nonmodel systems are becoming more feasible. Many of these systems may lack advanced genetic tools but are extremely valuable models in other biological fields. Here we report the development of expressed sequence tags (EST's) in an orthopteroid insect, a model for the study of neurobiology, speciation, and evolution. Results: We report the sequencing of 14,502 EST's from clones derived from a nerve cord cDNA library, and the subsequent construction of a Gene Index from these sequences, from the Hawaiian trigonidiine cricket Laupala kohalensis. The Gene Index contains 8607 unique sequences comprised of 2575 tentative consensus (TC) sequences and 6032 singletons. For each of the unique sequences, an attempt was made to assign a provisional annotation and to categorize its function using a Gene Ontology-based classification through a sequence-based comparison to known proteins. In addition, a set of unique 70 base pair oligomers that can be used for DNA microarrays was developed. All Gene Index information is posted at the DFCI Gene Indices web page. Conclusion: Orthopterans are models used to understand the neurophysiological basis of complex motor patterns such as flight and stridulation. The sequences presented in the cricket Gene Index will provide neurophysiologists with many genetic tools that have been largely absent in this field. The cricket Gene Index is one of only two gene indices to be developed in an evolutionary model system. Species within the genus Laupala have speciated recently, rapidly, and extensively. Therefore, the genes identified in the cricket Gene Index can be used to study the genomics of speciation. Furthermore, this gene index represents a significant EST resources for basal insects. As such, this resource is a valuable comparative tool for the understanding of invertebrate molecular evolution. The sequences presented here will provide much needed genomic resources for three distinct but overlapping fields of inquiry: neurobiology, speciation, and molecular evolution.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1471-2164-8-109en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1878485/pdf/en_US
dash.licenseLAA
dc.titleA Cricket Gene Index: A Genomic Resource for Studying Neurobiology, Speciation, and Molecular Evolutionen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBMC Genomicsen_US
dash.depositing.authorQuackenbush, John
dc.date.available2010-11-08T16:39:11Z
dash.affiliation.otherSPH^Biostatisticsen_US
dc.identifier.doi10.1186/1471-2164-8-109*
dash.contributor.affiliatedQuackenbush, John
dc.identifier.orcid0000-0002-2702-5879


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