Detection and Identification of NAP-2 as a Biomarker in Hepatitis B-Related Hepatocellular Carcinoma by Proteomic Approach

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Detection and Identification of NAP-2 as a Biomarker in Hepatitis B-Related Hepatocellular Carcinoma by Proteomic Approach

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Title: Detection and Identification of NAP-2 as a Biomarker in Hepatitis B-Related Hepatocellular Carcinoma by Proteomic Approach
Author: He, Min; Qin, Jian; Rong, Minhua; Jiang, Zhihua; Huang, Yuanjiao; Zhang, Zhiyong; Zhai, Rihong; Wei, Xiao; Wang, Qi

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Citation: He, Min, Jian Qin, Rihong Zhai, Xiao Wei, Qi Wang, Minhua Rong, Zhihua Jiang, Yuanjiao Huang, and Zhiyong Zhang. 2008. Detection and identification of NAP-2 as a biomarker in hepatitis B-related hepatocellular carcinoma by proteomic approach. Proteome Science 6:10.
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Abstract: Background: A lack of sensitive and specific biomarkers is a major reason for the high rate of Primary hepatocellular carcinoma (HCC)-related mortality. The aim of this study was to investigate potential proteomic biomarkers specific for HCC. Methods: 81 patients with hepatitis B-related HCC and 33 healthy controls were randomly divided into a training set (33 HCC, 33 controls) and a testing set (48 HCC, 33 controls). Serum proteomic profiles were measured using Surface-enhanced laser desorption/ionization-time-of-flight mass spectroscopy (SELDI-TOF-MS).) A classification tree was established by Biomarker Pattern Software (BPS). Candidate SELDI peaks were isolated by tricine-SDS-PAGE, identified by HPLC-MS/MS and validated by immunohistochemistry (IHC) in liver tissues. Results: A total of 6 proteomic peaks (3157.33 m/z, 4177.02 m/z, 4284.79 m/z, 4300.80 m/z, 7789.87 m/z, and 7984.14 m/z) were chosen by BPS to establish a classification tree with the highest discriminatory power in the training set. The sensitivity and specificity of this classification tree were 95.92%, and 100% respectively in the testing set. A candidate marker of about 7984 m/z was isolated and identified as neutrophil-activating peptide 2 (NAP-2). IHC staining showed that NAP-2 signals were positive in HCC tissues but negative in adjacent tissues. Conclusion: The NAP-2 may be a specific proteomic biomarker of hepatitis B-related HCC.
Published Version: doi:10.1186/1477-5956-6-10
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275230/pdf/
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Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4565605
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