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dc.contributor.authorFrench, Catherine A
dc.contributor.authorGroszer, Matthias
dc.contributor.authorPreece, Christopher
dc.contributor.authorCoupe, Anne-Marie
dc.contributor.authorRajewsky, Klaus
dc.contributor.authorFisher, Simon E
dc.date.accessioned2010-12-09T14:57:58Z
dc.date.issued2007
dc.identifier.citationFrench, Catherine A., Matthias Groszer, Christopher Preece, Anne-Marie Coupe, Klaus Rajewsky, and Simon E. Fisher. 2007. Generation of mice with a conditional null allele. Genesis 45(7): 440-446.en_US
dc.identifier.issn1526-954Xen_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4621601
dc.description.abstractDisruptions of the human FOXP2 gene cause problems with articulation of complex speech sounds, accompanied by impairment in many aspects of language ability. The FOXP2/Foxp2 transcription factor is highly similar in humans and mice, and shows a complex conserved expression pattern, with high levels in neuronal subpopulations of the cortex, striatum, thalamus, and cerebellum. In the present study we generated mice in which loxP sites flank exons 12–14 of Foxp2; these exons encode the DNA-binding motif, a key functional domain. We demonstrate that early global Cre-mediated recombination yields a null allele, as shown by loss of the loxP-flanked exons at the RNA level and an absence of Foxp2 protein. Homozygous null mice display severe motor impairment, cerebellar abnormalities and early postnatal lethality, consistent with other Foxp2 mutants. When crossed to transgenic lines expressing Cre protein in a spatially and/or temporally controlled manner, these conditional mice will provide new insights into the contributions of Foxp2 to distinct neural circuits, and allow dissection of roles during development and in the mature brain. genesis 45:440–446, 2007. Published 2007 Wiley-Liss, Inc.en_US
dc.language.isoen_USen_US
dc.publisherWiley-Blackwellen_US
dc.relation.isversionofdoi: 10.1002/dvg.20305en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2682329/pdf/en_US
dash.licenseMETA_ONLY
dc.subjectFoxp2en_US
dc.subjectconditional null alleleen_US
dc.subjectSox2-Creen_US
dc.subjectspeech and languageen_US
dc.subjectbrain developmenten_US
dc.titleGeneration of Mice with a Conditional Foxp2 Null Alleleen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalGenesis -- New York- Wiley Liss-en_US
dash.depositing.authorRajewsky, Klaus
dash.embargo.until10000-01-01
dash.affiliation.otherHMS^Pathologyen_US
dash.affiliation.otherHMS^Pediatrics-Children's Hospitalen_US
dc.identifier.doi10.1002/dvg.20305*
dash.contributor.affiliatedRajewsky, Klaus


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