Angiogenin Cleaves tRNA and Promotes Stress-Induced Translational Repression

DSpace/Manakin Repository

Angiogenin Cleaves tRNA and Promotes Stress-Induced Translational Repression

Citable link to this page


Title: Angiogenin Cleaves tRNA and Promotes Stress-Induced Translational Repression
Author: Yamasaki, Satoshi; Hu, Guo-fu; Ivanov, Pavel; Anderson, Paul

Note: Order does not necessarily reflect citation order of authors.

Citation: Yamasaki, Satoshi, Pavel Ivanov, Guo-fu Hu, and Paul Anderson. 2009. Angiogenin cleaves tRNA and promotes stress-induced translational repression. Journal of Cell Biology 185(1): 35-42.
Full Text & Related Files:
Abstract: Stress-induced phosphorylation of eIF2α inhibits global protein synthesis to conserve energy for repair of stress-induced damage. Stress-induced translational arrest is observed in cells expressing a nonphosphorylatable eIF2α mutant (S51A), which indicates the existence of an alternative pathway of translational control. In this paper, we show that arsenite, heat shock, or ultraviolet irradiation promotes transfer RNA (tRNA) cleavage and accumulation of tRNA-derived, stress-induced small RNAs (tiRNAs). We show that angiogenin, a secreted ribonuclease, is required for stress-induced production of tiRNAs. Knockdown of angiogenin, but not related ribonucleases, inhibits arsenite-induced tiRNA production and translational arrest. In contrast, knockdown of the angiogenin inhibitor RNH1 enhances tiRNA production and promotes arsenite-induced translational arrest. Moreover, recombinant angiogenin, but not RNase 4 or RNase A, induces tiRNA production and inhibits protein synthesis in the absence of exogenous stress. Finally, transfection of angiogenin-induced tiRNAs promotes phospho-eIF2α–independent translational arrest. Our results introduce angiogenin and tiRNAs as components of a phospho-eIF2α–independent stress response program.
Published Version: doi:10.1083/jcb.200811106
Other Sources:
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at
Citable link to this page:
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)


Search DASH

Advanced Search