Epstein–Barr Virus MicroRNAs Are Evolutionarily Conserved and Differentially Expressed
View/ Open
Author
Cai, Xuezhong
Schäfer, Alexandra
Lu, Shihua
Bilello, John P
Edwards, Rachel
Raab-Traub, Nancy
Cullen, Bryan R
Published Version
https://doi.org/10.1371/journal.ppat.0020023Metadata
Show full item recordCitation
Cai, Xuezhong, Alexandra Schäfer, Shihua Lu, John P. Bilello, Ronald C. Desrosiers, Rachel Edwards, Nancy Raab-Traub, and Bryan R. Cullen. 2006. Epstein–Barr Virus microRNAs are evolutionarily conserved and differentially expressed. PLoS Pathogens 2(3): e23.Abstract
The pathogenic lymphocryptovirus Epstein–Barr virus (EBV) is shown to express at least 17 distinct microRNAs (miRNAs) in latently infected cells. These are arranged in two clusters: 14 miRNAs are located in the introns of the viral BART gene while three are located adjacent to BHRF1. The BART miRNAs are expressed at high levels in latently infected epithelial cells and at lower, albeit detectable, levels in B cells. In contrast to the tissue-specific expression pattern of the BART miRNAs, the BHRF1 miRNAs are found at high levels in B cells undergoing stage III latency but are essentially undetectable in B cells or epithelial cells undergoing stage I or II latency. Induction of lytic EBV replication was found to enhance the expression of many, but not all, of these viral miRNAs. Rhesus lymphocryptovirus, which is separated from EBV by ≥13 million years of evolution, expresses at least 16 distinct miRNAs, seven of which are closely related to EBV miRNAs. Thus, lymphocryptovirus miRNAs are under positive selection and are likely to play important roles in the viral life cycle. Moreover, the differential regulation of EBV miRNA expression implies distinct roles during infection of different human tissues.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1409806/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:4621952
Collections
- HMS Scholarly Articles [17917]
Contact administrator regarding this item (to report mistakes or request changes)