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dc.contributor.authorMarselli, Lorella
dc.contributor.authorThorne, Jeffrey
dc.contributor.authorDahiya, Sonika
dc.contributor.authorSgroi, Dennis Charles
dc.contributor.authorSharma, Arun J.
dc.contributor.authorBonner-Weir, Susan
dc.contributor.authorWeir, Gordon Campbell
dc.contributor.authorMarchetti, Piero
dc.date.accessioned2010-12-21T15:42:51Z
dc.date.issued2010
dc.identifier.citationMarselli, Lorella, Jeffrey Thorne, Sonika Dahiya, Dennis C. Sgroi, Arun Sharma, Susan Bonner-Weir, Piero Marchetti, and Gordon C. Weir. 2010. Gene expression profiles of beta-cell enriched tissue obtained by laser capture microdissection from subjects with type 2 diabetes. PLoS ONE 5(7): e11499.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4632525
dc.description.abstractBackground: Changes in gene expression in pancreatic beta-cells from type 2 diabetes (T2D) should provide insights into their abnormal insulin secretion and turnover. Methodology/Principal Findings: Frozen sections were obtained from cadaver pancreases of 10 control and 10 T2D human subjects. Beta-cell enriched samples were obtained by laser capture microdissection (LCM). RNA was extracted, amplified and subjected to microarray analysis. Further analysis was performed with DNA-Chip Analyzer (dChip) and Gene Set Enrichment Analysis (GSEA) software. There were changes in expression of genes linked to glucotoxicity. Evidence of oxidative stress was provided by upregulation of several metallothionein genes. There were few changes in the major genes associated with cell cycle, apoptosis or endoplasmic reticulum stress. There was differential expression of genes associated with pancreatic regeneration, most notably upregulation of members of the regenerating islet gene (REG) family and metalloproteinase 7 (MMP7). Some of the genes found in GWAS studies to be related to T2D were also found to be differentially expressed. IGF2BP2, TSPAN8, and HNF1B (TCF2) were upregulated while JAZF1 and SLC30A8 were downregulated. Conclusions/Significance: This study made possible by LCM has identified many novel changes in gene expression that enhance understanding of the pathogenesis of T2D.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0011499en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2903480/pdf/en_US
dash.licenseLAA
dc.subjectmolecular biologyen_US
dc.subjectcell biologyen_US
dc.subjectgene expressionen_US
dc.subjectdiabetes and endocrinologyen_US
dc.subjecttype 2 diabetesen_US
dc.titleGene Expression Profiles of Beta-Cell Enriched Tissue Obtained by Laser Capture Microdissection from Subjects with Type 2 Diabetesen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorWeir, Gordon Campbell
dc.date.available2010-12-21T15:42:51Z
dash.affiliation.otherHMS^Pathologyen_US
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dc.identifier.doi10.1371/journal.pone.0011499*
dash.contributor.affiliatedSharma, Arun J.
dash.contributor.affiliatedSgroi, Dennis
dash.contributor.affiliatedBonner-Weir, Susan
dash.contributor.affiliatedWeir, Gordon


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