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dc.contributor.authorCohen, Shenhav Orit
dc.contributor.authorBrault, Jeffrey J.
dc.contributor.authorGygi, Steven P.
dc.contributor.authorGlass, David Jonathan
dc.contributor.authorValenzuela, David M.
dc.contributor.authorGartner, Carlos
dc.contributor.authorLatres, Esther
dc.contributor.authorGoldberg, Alfred L.
dc.date.accessioned2011-02-05T14:00:39Z
dc.date.issued2009
dc.identifier.citationCohen, Shenhav O., Jeffrey J. Brault, Steven P. Gygi, David J. Glass, David M. Valenzuela, Carlos Gartner, Esther Latres, and Alfred L. Goldberg. 2009. During muscle atrophy, thick, but not thin, filament components are degraded by MuRF1-dependent ubiquitylation. Journal of Cell Biology 185(6): 1083-1095.en_US
dc.identifier.issn0021-9525en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4706108
dc.description.abstractLoss of myofibrillar proteins is a hallmark of atrophying muscle. Expression of muscle RING-finger 1 (MuRF1), a ubiquitin ligase, is markedly induced during atrophy, and MuRF1 deletion attenuates muscle wasting. We generated mice expressing a Ring-deletion mutant MuRF1, which binds but cannot ubiquitylate substrates. Mass spectrometry of the bound proteins in denervated muscle identified many myofibrillar components. Upon denervation or fasting, atrophying muscles show a loss of myosin-binding protein C (MyBP-C) and myosin light chains 1 and 2 (MyLC1 and MyLC2) from the myofibril, before any measurable decrease in myosin heavy chain (MyHC). Their selective loss requires MuRF1. MyHC is protected from ubiquitylation in myofibrils by associated proteins, but eventually undergoes MuRF1-dependent degradation. In contrast, MuRF1 ubiquitylates MyBP-C, MyLC1, and MyLC2, even in myofibrils. Because these proteins stabilize the thick filament, their selective ubiquitylation may facilitate thick filament disassembly. However, the thin filament components decreased by a mechanism not requiring MuRF1.en_US
dc.language.isoen_USen_US
dc.publisherRockefeller University Pressen_US
dc.relation.isversionofdoi:10.1083/jcb.200901052en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711608/pdf/en_US
dash.licenseLAA
dc.titleDuring Muscle Atrophy, Thick, but not Thin, Filament Components are Degraded by MuRF1-Dependent Ubiquitylationen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalJournal of Cell Biologyen_US
dash.depositing.authorGoldberg, Alfred L.
dc.date.available2011-02-05T14:00:39Z
dash.affiliation.otherHMS^Cell Biologyen_US
dash.affiliation.otherHMS^Cell Biologyen_US
dc.identifier.doi10.1083/jcb.200901052*
dash.contributor.affiliatedCohen, Shenhav Orit
dash.contributor.affiliatedGlass, David
dash.contributor.affiliatedGoldberg, Alfred
dash.contributor.affiliatedGygi, Steven


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