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dc.contributor.authorMcBride, Shonna M.
dc.contributor.authorFischetti, Vincent A.
dc.contributor.authorLeBlanc, Donald J.
dc.contributor.authorMoellering, Robert Charles
dc.contributor.authorGilmore, Michael S.
dc.date.accessioned2011-02-07T17:19:21Z
dc.date.issued2007
dc.identifier.citationMcBride, Shonna M., Vincent A. Fischetti, Donald J. LeBlanc, Robert C. Moellering, and Michael S. Gilmore. 2007. Genetic Diversity among Enterococcus faecalis. PLoS ONE 2(7).en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4706589
dc.description.abstractEnterococcus faecalis, a ubiquitous member of mammalian gastrointestinal flora, is a leading cause of nosocomial infections and a growing public health concern. The enterococci responsible for these infections are often resistant to multiple antibiotics and have become notorious for their ability to acquire and disseminate antibiotic resistances. In the current study, we examined genetic relationships among 106 strains of E. faecalis isolated over the past 100 years, including strains identified for their diversity and used historically for serotyping, strains that have been adapted for laboratory use, and isolates from previously described E. faecalis infection outbreaks. This collection also includes isolates first characterized as having novel plasmids, virulence traits, antibiotic resistances, and pathogenicity island (PAI) components. We evaluated variation in factors contributing to pathogenicity, including toxin production, antibiotic resistance, polymorphism in the capsule (cps) operon, pathogenicity island (PAI) gene content, and other accessory factors. This information was correlated with multi-locus sequence typing (MLST) data, which was used to define genetic lineages. Our findings show that virulence and antibiotic resistance traits can be found within many diverse lineages of E. faecalis. However, lineages have emerged that have caused infection outbreaks globally, in which several new antibiotic resistances have entered the species, and in which virulence traits have converged. Comparing genomic hybridization profiles, using a microarray, of strains identified by MLST as spanning the diversity of the species, allowed us to identify the core E. faecalis genome as consisting of an estimated 2057 unique genes.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi://10.1371/journal.pone.0000582en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1899230/pdf/en_US
dash.licenseLAA
dc.subjectmicrobiologyen_US
dc.subjectmicrobial evolution and genomicsen_US
dc.subjectmedical microbiologyen_US
dc.subjectgenetics and genomicsen_US
dc.subjectpopulation geneticsen_US
dc.subjectmicrobial evolution and genomicsen_US
dc.subjectcomparative genomicsen_US
dc.titleGenetic Diversity among Enterococcus faecalisen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorMoellering, Robert Charles
dc.date.available2011-02-07T17:19:21Z
dash.affiliation.otherHMS^Medicine- Beth Israel-Deaconessen_US
dash.affiliation.otherHMS^Ophthalmologyen_US
dc.identifier.doi10.1371/journal.pone.0000582*
dash.contributor.affiliatedMoellering, Robert Charles
dash.contributor.affiliatedGilmore, Michael


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