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dc.contributor.authorWhitney, James B.
dc.contributor.authorWainberg, Mark A
dc.date.accessioned2011-02-07T17:30:05Z
dc.date.issued2006
dc.identifier.citationWhitney, James B, and Mark A Wainberg. 2006. Impaired RNA incorporation and dimerization in live attenuated leader-variants of SIV. Retrovirology 3: 96.en_US
dc.identifier.issn1742-4690en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4706591
dc.description.abstractBackground: The 5' untranslated region (UTR) or leader sequence of simian immunodeficiency virus (SIVmac239) is multifunctional and harbors the regulatory elements for viral replication, persistence, gene translation, expression, and the packaging and dimerization of viral genomic RNA (vRNA). We have constructed a series of deletions in the SIVmac239 leader sequence in order to determine the involvement of this region in both the packaging and dimerization of viral genomic RNA. We also assessed the impact of these deletions upon viral infectiousness, replication kinetics and gene expression in cell lines and monkey peripheral blood mononuclear cells (PBMC). Results: Regions on both sides of the major splice donor (SD) were found to be necessary for the efficiency and specificity of viral genome packaging. However, stem-loop1 is critical for both RNA encapsidation and dimerization. Downstream elements between the splice donor and the initiation site of SIV-Gag have additive effects on RNA packaging and contribute to a lesser degree to RNA dimerization. The targeted disruption of structures on both sides of the SD also severely impacts viral infectiousness, gene expression and replication in both CEMx174 cells and rhesus PBMC. Conclusion: In the leader region of SIVmac239, stem-loop1 functions as the primary determinant for both RNA encapsidation and dimerization. Downstream elements between the splice donor and the translational initiation site of SIV-Gag are classified as secondary determinants and play a role in dimerization. Collectively, these data signify a linkage between the primary encapsidation determinant of SIVmac239 and RNA dimerization.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi://10.1186/1742-4690-3-96en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1766366/pdf/en_US
dash.licenseLAA
dc.titleImpaired RNA Incorporation and Dimerization in Live Attenuated Leader-variants of SIVmac239en_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalRetrovirologyen_US
dash.depositing.authorWhitney, James B.
dc.date.available2011-02-07T17:30:05Z
dash.affiliation.otherHMS^Medicine- Beth Israel-Deaconessen_US
dc.identifier.doi10.1186/1742-4690-3-96*
dash.contributor.affiliatedWhitney, James


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