Global Gene Expression of Prochlorococcus ecotypes in Response to Changes in Nitrogen Availability

Global Gene Expression of Prochlorococcus ecotypes in Response to Changes in Nitrogen Availability

DSpace/Manakin Repository

Global Gene Expression of Prochlorococcus ecotypes in Response to Changes in Nitrogen Availability

dc.contributor.author	Lindell, Debbie
dc.contributor.author	Johnson, Zackary I
dc.contributor.author	Rector, Trent
dc.contributor.author	Chisholm, Sallie W
dc.contributor.author	Tolonen, Andrew
dc.contributor.author	Aach, John Dennis
dc.contributor.author	Steen, Robert G.
dc.date.accessioned	2011-02-15T00:17:24Z
dc.date.issued	2006
dc.identifier.citation	Tolonen, Andrew C., John Aach, Debbie Lindell, Zackary I. Johnson, Trent Rector, Robert Steen, George M. Church, and Sallie W. Chisholm. 2006. Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability. Molecular Systems Biology 2: 53.	en_US
dc.identifier.issn	1744-4292	en_US
dc.identifier.uri	http://nrs.harvard.edu/urn-3:HUL.InstRepos:4725512
dc.description.abstract	Nitrogen (N) often limits biological productivity in the oceanic gyres where Prochlorococcus is the most abundant photosynthetic organism. The Prochlorococcus community is composed of strains, such as MED4 and MIT9313, that have different N utilization capabilities and that belong to ecotypes with different depth distributions. An interstrain comparison of how Prochlorococcus responds to changes in ambient nitrogen is thus central to understanding its ecology. We quantified changes in MED4 and MIT9313 global mRNA expression, chlorophyll fluorescence, and photosystem II photochemical efficiency (Fv/Fm) along a time series of increasing N starvation. In addition, the global expression of both strains growing in ammonium-replete medium was compared to expression during growth on alternative N sources. There were interstrain similarities in N regulation such as the activation of a putative NtcA regulon during N stress. There were also important differences between the strains such as in the expression patterns of carbon metabolism genes, suggesting that the two strains integrate N and C metabolism in fundamentally different ways.	en_US
dc.language.iso	en_US	en_US
dc.publisher	European Molecular Biology Organization	en_US
dc.relation.isversionof	doi:10.1038/msb4100087	en_US
dc.relation.hasversion	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1682016/pdf/	en_US
dash.license	LAA
dc.subject	cyanobacteria	en_US
dc.subject	interstrain	en_US
dc.subject	nitrogen	en_US
dc.subject	Prochlorococcus	en_US
dc.subject	transcription	en_US
dc.title	Global Gene Expression of Prochlorococcus ecotypes in Response to Changes in Nitrogen Availability	en_US
dc.type	Journal Article	en_US
dc.description.version	Version of Record	en_US
dc.relation.journal	Molecular Systems Biology	en_US
dash.depositing.author	Aach, John Dennis
dc.date.available	2011-02-15T00:17:24Z
dash.affiliation.other	HMS^Genetics	en_US
dash.affiliation.other	HMS^Genetics	en_US
dash.affiliation.other	HMS^Health Sciences and Technology	en_US
dash.affiliation.other	HMS^Genetics	en_US