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dc.contributor.authorPaskaleva, Elena E
dc.contributor.authorLin, Xudong
dc.contributor.authorCotter, Robin
dc.contributor.authorKlein, Michael T
dc.contributor.authorRoberge, Emily
dc.contributor.authorYu, Er K
dc.contributor.authorClark, Bruce
dc.contributor.authorVeille, Jean-Claude
dc.contributor.authorLiu, Yanze
dc.contributor.authorCanki, Mario
dc.contributor.authorLee, David Marvin
dc.contributor.authorLi, Wen
dc.date.accessioned2011-02-18T17:25:12Z
dc.date.issued2006
dc.identifier.citationPaskaleva, Elena E., Xudong Lin, Wen Li, Robin Cotter, Michael T. Klein, Emily Roberge, Er K. Yu, et al. 2006. Inhibition of highly productive HIV-1 infection in T cells, primary human macrophages, microglia, and astrocytes by Sargassum fusiforme. AIDS Research and Therapy 3: 15.en_US
dc.identifier.issn1742-6405en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4727708
dc.description.abstractBackground: The high rate of HIV-1 mutation and increasing resistance to currently available antiretroviral (ART) therapies highlight the need for new antiviral agents. Products derived from natural sources have been shown to inhibit HIV-1 replication during various stages of the virus life cycle, and therefore represent a potential source of novel therapeutic agents. To expand our arsenal of therapeutics against HIV-1 infection, we investigated aqueous extract from Sargassum fusiforme (S. fusiforme) for ability to inhibit HIV-1 infection in the periphery, in T cells and human macrophages, and for ability to inhibit in the central nervous system (CNS), in microglia and astrocytes. Results: S. fusiforme extract blocked HIV-1 infection and replication by over 90% in T cells, human macrophages and microglia, and it also inhibited pseudotyped HIV-1 (VSV/NL4-3) infection in human astrocytes by over 70%. Inhibition was mediated against both CXCR4 (X4) and CCR5 (R5)-tropic HIV-1, was dose dependant and long lasting, did not inhibit cell growth or viability, was not toxic to cells, and was comparable to inhibition by the nucleoside analogue 2', 3'-didoxycytidine (ddC). S. fusiforme treatment blocked direct cell-to-cell infection spread. To investigate at which point of the virus life cycle this inhibition occurs, we infected T cells and CD4-negative primary human astrocytes with HIV-1 pseudotyped with envelope glycoprotein of vesicular stomatitis virus (VSV), which bypasses the HIV receptor requirements. Infection by pseudotyped HIV-1 (VSV/NL4-3) was also inhibited in a dose dependant manner, although up to 57% less, as compared to inhibition of native NL4-3, indicating post-entry interferences. Conclusion: This is the first report demonstrating S. fusiforme to be a potent inhibitor of highly productive HIV-1 infection and replication in T cells, in primary human macrophages, microglia, and astrocytes. Results with VSV/NL4-3 infection, suggest inhibition of both entry and post-entry events of the virus life cycle. Absence of cytotoxicity and high viability of treated cells also suggest that S. fusiforme is a potential source of novel naturally occurring antiretroviral compounds that inhibit HIV-1 infection and replication at more than one site of the virus life cycle.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi: 10.1186/1742-6405-3-15en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1481599/pdf/en_US
dash.licenseLAA
dc.titleInhibition of Highly Productive HIV-1 Infection in T Cells, Primary Human Macrophages, Microglia, and Astrocytes by Sargassum fusiformeen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalAIDS Research and Therapyen_US
dash.depositing.authorLee, David Marvin
dc.date.available2011-02-18T17:25:12Z
dash.affiliation.otherHMS^Medicine-Brigham and Women's Hospitalen_US
dc.identifier.doi10.1186/1742-6405-3-15*
dash.authorsorderedfalse
dash.contributor.affiliatedLee, David Marvin


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