dc.contributor.author | Grey, Finn | |
dc.contributor.author | Meyers, Heather | |
dc.contributor.author | White, Elizabeth | |
dc.contributor.author | Spector, Deborah H | |
dc.contributor.author | Nelson, Jay | |
dc.date.accessioned | 2011-02-22T17:43:47Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Grey, Finn, Heather Meyers, Elizabeth A. White, Deborah H. Spector, and Jay Nelson. 2007. A human cytomegalovirus-encoded microRNA regulates expression of multiple viral genes involved in replication. PLoS Pathogens 3(11): e163. | en_US |
dc.identifier.issn | 1553-7366 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4729253 | |
dc.description.abstract | Although multiple studies have documented the expression of over 70 novel virus-encoded microRNAs (miRNAs), the targets and functions of most of these regulatory RNA species are unknown. In this study a comparative bioinformatics approach was employed to identify potential human cytomegalovirus (HCMV) mRNA targets of the virus-encoded miRNA miR-UL112-1. Bioinformatics analysis of the known HCMV mRNA 3′ untranslated regions (UTRs) revealed 14 potential viral transcripts that were predicted to contain functional target sites for miR-UL112-1. The potential target sites were screened using luciferase reporters that contain the HCMV 3′UTRs in co-transfection assays with miR-UL112-1. Three of the 14 HCMV miRNA targets were validated, including the major immediate early gene encoding IE72 (UL123, IE1), UL112/113, and UL120/121. Further analysis of IE72 regulation by miR-UL112-1 with clones encoding the complete major immediate early region revealed that the IE72 3′UTR target site is necessary and sufficient to direct miR-UL112-1-specific inhibition of expression in transfected cells. In addition, miR-UL112-1 regulation is mediated through translational inhibition rather than RNA degradation. Premature expression of miR-UL112-1 during HCMV infection resulted in a significant decrease in genomic viral DNA levels, suggesting a functional role for miR-UL112-1 in regulating the expression of genes involved in viral replication. This study demonstrates the ability of a viral miRNA to regulate multiple viral genes. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.isversionof | doi:10.1371/journal.ppat.0030163 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2048532/pdf/ | en_US |
dash.license | LAA | |
dc.title | A Human Cytomegalovirus-Encoded microRNA Regulates Expression of Multiple Viral Genes Involved in Replication | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | PLoS Pathogens | en_US |
dash.depositing.author | White, Elizabeth | |
dc.date.available | 2011-02-22T17:43:47Z | |
dash.affiliation.other | HMS^Pathology | en_US |
dc.identifier.doi | 10.1371/journal.ppat.0030163 | * |
dash.contributor.affiliated | White, Elizabeth A. | |