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dc.contributor.authorHsu, Carolyn T
dc.contributor.authorGanong, Amanda L
dc.contributor.authorReinap, Barbara
dc.contributor.authorMourelatos, Zafiria
dc.contributor.authorHuebner, Johannes
dc.contributor.authorWang, Ying Ying
dc.date.accessioned2011-03-02T00:16:57Z
dc.date.issued2006
dc.identifier.citationHsu, Carolyn T., Amanda L. Ganong, Barbara Reinap, Zafiria Mourelatos, Johannes Huebner, and Julia Y. Wang. 2006. Immunochemical characterization of polysaccharide antigens from six clinical strains of enterococci. BMC Microbiology 6: 62.en_US
dc.identifier.issn1471-2180en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4734532
dc.description.abstractBackground: Enterococci have become major nosocomial pathogens due to their intrinsic and acquired resistance to a broad spectrum of antibiotics. Their increasing drug resistance prompts us to search for prominent antigens to develop vaccines against enterococci. Given the success of polysaccharide-based vaccines against various bacterial pathogens, we isolated and characterized the immunochemical properties of polysaccharide antigens from five strains of Enterococcus faecalis and one strain of vancomycin-resistant E. faecium. Results: We cultured large batches of each strain, isolated sufficient quantities of polysaccharides, analyzed their chemical structures, and compared their antigenic specificity. Three classes of polysaccharides were isolated from each strain, including a polyglucan, a teichoic acid, and a heteroglycan composed of rhamnose, glucose, galactose, mannosamine, and glucosamine. The polyglucans from all six strains are identical and appear to be dextran. Yields of the teichoic acids were generally low. The most abundant polysaccharides are the heteroglycans. The six heteroglycans are structurally different as evidenced by NMR spectroscopy. They also differ in their antigenic specificities as revealed by competitive ELISA. The heteroglycans are not immunogenic by themselves but conjugation to protein carriers significantly enhanced their ability to induce antibodies. Conclusion: The six clinical strains of enterococci express abundant, strain-specific cell-surface heteroglycans. These polysaccharides may provide a molecular basis for serological typing of enterococcal strains and antigens for the development of vaccines against multi-drug resistant enterococci.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1471-2180-6-62en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1538600/pdf/en_US
dash.licenseLAA
dc.titleImmunochemical Characterization of Polysaccharide Antigens from Six Clinical Strains of Enterococcien_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBMC Microbiologyen_US
dash.depositing.authorWang, Ying Ying
dc.date.available2011-03-02T00:16:57Z
dash.affiliation.otherHMS^Medicine- Beth Israel-Deaconessen_US
dc.identifier.doi10.1186/1471-2180-6-62*
dash.contributor.affiliatedWang, Ying Ying


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