Immunochemical Characterization of Polysaccharide Antigens from Six Clinical Strains of Enterococci

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Immunochemical Characterization of Polysaccharide Antigens from Six Clinical Strains of Enterococci

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dc.contributor.author Hsu, Carolyn T
dc.contributor.author Ganong, Amanda L
dc.contributor.author Reinap, Barbara
dc.contributor.author Mourelatos, Zafiria
dc.contributor.author Huebner, Johannes
dc.contributor.author Wang, Ying Ying
dc.date.accessioned 2011-03-02T00:16:57Z
dc.date.issued 2006
dc.identifier.citation Hsu, Carolyn T., Amanda L. Ganong, Barbara Reinap, Zafiria Mourelatos, Johannes Huebner, and Julia Y. Wang. 2006. Immunochemical characterization of polysaccharide antigens from six clinical strains of enterococci. BMC Microbiology 6: 62. en_US
dc.identifier.issn 1471-2180 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4734532
dc.description.abstract Background: Enterococci have become major nosocomial pathogens due to their intrinsic and acquired resistance to a broad spectrum of antibiotics. Their increasing drug resistance prompts us to search for prominent antigens to develop vaccines against enterococci. Given the success of polysaccharide-based vaccines against various bacterial pathogens, we isolated and characterized the immunochemical properties of polysaccharide antigens from five strains of Enterococcus faecalis and one strain of vancomycin-resistant E. faecium. Results: We cultured large batches of each strain, isolated sufficient quantities of polysaccharides, analyzed their chemical structures, and compared their antigenic specificity. Three classes of polysaccharides were isolated from each strain, including a polyglucan, a teichoic acid, and a heteroglycan composed of rhamnose, glucose, galactose, mannosamine, and glucosamine. The polyglucans from all six strains are identical and appear to be dextran. Yields of the teichoic acids were generally low. The most abundant polysaccharides are the heteroglycans. The six heteroglycans are structurally different as evidenced by NMR spectroscopy. They also differ in their antigenic specificities as revealed by competitive ELISA. The heteroglycans are not immunogenic by themselves but conjugation to protein carriers significantly enhanced their ability to induce antibodies. Conclusion: The six clinical strains of enterococci express abundant, strain-specific cell-surface heteroglycans. These polysaccharides may provide a molecular basis for serological typing of enterococcal strains and antigens for the development of vaccines against multi-drug resistant enterococci. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-2180-6-62 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1538600/pdf/ en_US
dash.license LAA
dc.title Immunochemical Characterization of Polysaccharide Antigens from Six Clinical Strains of Enterococci en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Microbiology en_US
dash.depositing.author Wang, Ying Ying
dc.date.available 2011-03-02T00:16:57Z
dash.affiliation.other HMS^Medicine- Beth Israel-Deaconess en_US

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