Preparation of Large Monodisperse Vesicles

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Preparation of Large Monodisperse Vesicles

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Title: Preparation of Large Monodisperse Vesicles
Author: Zhu, Ting; Szostak, Jack William

Note: Order does not necessarily reflect citation order of authors.

Citation: Zhu, Ting F., and Jack W. Szostak. 2009. Preparation of large monodisperse vesicles. PLoS ONE 4(4).
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Abstract: Preparation of monodisperse vesicles is important both for research purposes and for practical applications. While the extrusion of vesicles through small pores (~100 nm in diameter) results in relatively uniform populations of vesicles, extrusion to larger sizes results in very heterogeneous populations of vesicles. Here we report a simple method for preparing large monodisperse multilamellar vesicles through a combination of extrusion and large-pore dialysis. For example, extrusion of polydisperse vesicles through 5-µm-diameter pores eliminates vesicles larger than 5 µm in diameter. Dialysis of extruded vesicles against 3-µm-pore-size polycarbonate membranes eliminates vesicles smaller than 3 µm in diameter, leaving behind a population of monodisperse vesicles with a mean diameter of ~4 µm. The simplicity of this method makes it an effective tool for laboratory vesicle preparation with potential applications in preparing large monodisperse liposomes for drug delivery.
Published Version: doi://10.1371/journal.pone.0005009
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2661144/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4737685
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