Preparation of Large Monodisperse Vesicles

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Preparation of Large Monodisperse Vesicles

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Title: Preparation of Large Monodisperse Vesicles
Author: Zhu, Ting; Szostak, Jack William

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Citation: Zhu, Ting F., and Jack W. Szostak. 2009. Preparation of large monodisperse vesicles. PLoS ONE 4(4).
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Abstract: Preparation of monodisperse vesicles is important both for research purposes and for practical applications. While the extrusion of vesicles through small pores (~100 nm in diameter) results in relatively uniform populations of vesicles, extrusion to larger sizes results in very heterogeneous populations of vesicles. Here we report a simple method for preparing large monodisperse multilamellar vesicles through a combination of extrusion and large-pore dialysis. For example, extrusion of polydisperse vesicles through 5-µm-diameter pores eliminates vesicles larger than 5 µm in diameter. Dialysis of extruded vesicles against 3-µm-pore-size polycarbonate membranes eliminates vesicles smaller than 3 µm in diameter, leaving behind a population of monodisperse vesicles with a mean diameter of ~4 µm. The simplicity of this method makes it an effective tool for laboratory vesicle preparation with potential applications in preparing large monodisperse liposomes for drug delivery.
Published Version: doi://10.1371/journal.pone.0005009
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