Mechanism of Sequence-Specific Template Binding by the DNA Primase of Bacteriophage T7
DSpace/Manakin Repository
Mechanism of Sequence-Specific Template Binding by the DNA Primase of Bacteriophage T7
| Title: | Mechanism of Sequence-Specific Template Binding by the DNA Primase of Bacteriophage T7 |
| Author: |
Hamdan, Samir M.; Lee, Seung-Joo; Zhu, Bin; Richardson, Charles C.
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Lee, Seung-Joo, Bin Zhu, Samir M. Hamdan, and Charles C. Richardson. 2010. Mechanism of sequence-specific template binding by the DNA primase of bacteriophage T7. Nucleic Acids Research 38(13): 4372-4383. |
| Full Text & Related Files: |
2910064.pdf (3.083Mb; PDF) 
|
| Abstract: | DNA primases catalyze the synthesis of the oligoribonucleotides required for the initiation of lagging strand DNA synthesis. Biochemical studies have elucidated the mechanism for the sequence-specific synthesis of primers. However, the physical interactions of the primase with the DNA template to explain the basis of specificity have not been demonstrated. Using a combination of surface plasmon resonance and biochemical assays, we show that T7 DNA primase has only a slightly higher affinity for DNA containing the primase recognition sequence (5′-TGGTC-3′) than for DNA lacking the recognition site. However, this binding is drastically enhanced by the presence of the cognate Nucleoside triphosphates (NTPs), Adenosine triphosphate (ATP) and Cytosine triphosphate (CTP) that are incorporated into the primer, pppACCA. Formation of the dimer, pppAC, the initial step of sequence-specific primer synthesis, is not sufficient for the stable binding. Preformed primers exhibit significantly less selective binding than that observed with ATP and CTP. Alterations in subdomains of the primase result in loss of selective DNA binding. We present a model in which conformational changes induced during primer synthesis facilitate contact between the zinc-binding domain and the polymerase domain. |
| Published Version: | doi:10.1093/nar/gkq205 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910064/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4738029 |
| Downloads of this work: |
This item appears in the following Collection(s)
-
HMS Scholarly Articles [16875]
Contact administrator regarding this item (to report mistakes or request changes)
