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dc.contributor.authorDoan, Thierry
dc.contributor.authorMorlot, Cecile
dc.contributor.authorMeisner, Jeffrey
dc.contributor.authorSerrano, Monica
dc.contributor.authorHenriques, Adriano O.
dc.contributor.authorMoran, Charles P. Jr.
dc.contributor.authorRudner, David Z.
dc.date.accessioned2011-03-16T14:23:13Z
dc.date.issued2009
dc.identifier.citationDoan, Thierry, Cecile Morlot, Jeffrey Meisner, Monica Serrano, Adriano O. Henriques, Charles P. Moran, Jr., and David Z. Rudner. 2009. Novel secretion apparatus maintains spore integrity and developmental gene expression in Bacillus subtilis. PLoS Genetics 5(7): e1000566.en_US
dc.identifier.issn1553-7390en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4742713
dc.description.abstractSporulation in Bacillus subtilis involves two cells that follow separate but coordinately regulated developmental programs. Late in sporulation, the developing spore (the forespore) resides within a mother cell. The regulation of the forespore transcription factor σG that acts at this stage has remained enigmatic. σG activity requires eight mother-cell proteins encoded in the spoIIIA operon and the forespore protein SpoIIQ. Several of the SpoIIIA proteins share similarity with components of specialized secretion systems. One of them resembles a secretion ATPase and we demonstrate that the ATPase motifs are required for σG activity. We further show that the SpoIIIA proteins and SpoIIQ reside in a multimeric complex that spans the two membranes surrounding the forespore. Finally, we have discovered that these proteins are all required to maintain forespore integrity. In their absence, the forespore develops large invaginations and collapses. Importantly, maintenance of forespore integrity does not require σG. These results support a model in which the SpoIIIA-SpoIIQ proteins form a novel secretion apparatus that allows the mother cell to nurture the forespore, thereby maintaining forespore physiology and σG activity during spore maturation.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi://10.1371/journal.pgen.1000566en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703783/pdf/en_US
dash.licenseLAA
dc.subjectcell biologyen_US
dc.subjectcell signalingen_US
dc.subjectdevelopmental molecular mechanismsen_US
dc.subjectgene expressionen_US
dc.subjectmicrobial growth and developmenten_US
dc.subjectdevelopmental biologyen_US
dc.subjectcell differentiationen_US
dc.subjectmicrobial growth and developmenten_US
dc.subjectgenetics and genomicsen_US
dc.subjectgene expressionen_US
dc.subjectgene functionen_US
dc.subjectmicrobiologyen_US
dc.subjectmicrobial growth and developmenten_US
dc.subjectmolecular biologyen_US
dc.subjectpost-translational regulation of gene expressionen_US
dc.titleNovel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilisen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Geneticsen_US
dash.depositing.authorRudner, David Z.
dc.date.available2011-03-16T14:23:13Z
dash.affiliation.otherHMS^Microbiology and Molecular Geneticsen_US
dc.identifier.doi10.1371/journal.pgen.1000566*
dash.contributor.affiliatedRudner, David


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