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dc.contributor.authorBerger, Cedric R.
dc.contributor.authorFigueiredo, Jose-Luiz
dc.contributor.authorSwirski, Filip K.
dc.contributor.authorMempel, Thorsten Roman
dc.contributor.authorVon Andrian, Ulrich H.
dc.contributor.authorPittet, Mikael J.
dc.contributor.authorWeissleder, Ralph
dc.date.accessioned2011-03-19T01:08:22Z
dc.date.issued2007
dc.identifier.citationSwirski, Filip K., Cedric R. Berger, Jose-Luiz Figueiredo, Thorsten R. Mempel, Ulrich H. von Andrian, Mikael J. Pittet, and Ralph Weissleder. 2007. A Near-Infrared Cell Tracker Reagent for Multiscopic In Vivo Imaging and Quantification of Leukocyte Immune Responses. PLoS ONE 2(10): e1075.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4745724
dc.description.abstractThe complexity of the tumor microenvironment necessitates that cell behavior is studied in a broad, multi-scale context. Although tomographic and microscopy-based far and near infrared fluorescence (NIRF, >650 nm) imaging methods offer high resolution, sensitivity, and depth penetration, there has been a lack of optimized NIRF agents to label and track cells in their native environments at different scales. In this study we labeled mammalian leukocytes with VivoTag 680 (VT680), an amine reactive N-hydroxysuccinimide (NHS) ester of a (benz) indolium-derived far red fluorescent probe. We show that VT680 diffuses into leukocytes within minutes, covalently binds to cellular components, remains internalized for days in vitro and in vivo, and does not transfer fluorescence to adjacent cells. It is biocompatible, keeps cells fully functional, and fluoresces at high intensities. In a tumor model of cytotoxic T lymphocyte (CTL) immunotherapy, we track and quantify VT680-labeled cells longitudinally at the whole-body level with fluorescence-mediated molecular tomography (FMT), within tissues at single cell resolutions by multiphoton and confocal intravital microscopy, and ex vivo by flow cytometry. Thus, this approach is suitable to monitor cells at multiple resolutions in real time in their native environments by NIR-based fluorescence imaging.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0001075en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034600/pdf/en_US
dash.licenseLAA
dc.subjectradiology and medical imagingen_US
dc.subjectimmunologyen_US
dc.subjectimmune responseen_US
dc.subjectimmunomodulationen_US
dc.subjectleukocyte activationen_US
dc.titleA Near-Infrared Cell Tracker Reagent for Multiscopic In Vivo Imaging and Quantification of Leukocyte Immune Responsesen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorSwirski, Filip K.
dc.date.available2011-03-19T01:08:22Z
dash.affiliation.otherHMS^Radiology-Massachusetts General Hospitalen_US
dash.affiliation.otherHMS^Radiology-Massachusetts General Hospitalen_US
dash.affiliation.otherHMS^Radiology-Massachusetts General Hospitalen_US
dash.affiliation.otherHMS^Systems Biologyen_US
dc.identifier.doi10.1371/journal.pone.0001075*
dash.authorsorderedfalse
dash.contributor.affiliatedPittet, Mikael
dash.contributor.affiliatedMempel, Thorsten
dash.contributor.affiliatedvon Andrian-Werburg, Ulrich
dash.contributor.affiliatedSwirski, Filip
dash.contributor.affiliatedWeissleder, Ralph


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