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dc.contributor.authorViswanathan, Karthik
dc.contributor.authorKoh, Xiaoying
dc.contributor.authorChandrasekaran, Aarthi
dc.contributor.authorPappas, Claudia
dc.contributor.authorRaman, Rahul
dc.contributor.authorSrinivasan, Aravind
dc.contributor.authorShriver, Zachary
dc.contributor.authorTumpey, Terrence M.
dc.contributor.authorSasisekharan, Ram
dc.date.accessioned2011-03-19T07:51:40Z
dc.date.issued2010
dc.identifier.citationViswanathan, Karthik, Xiaoying Koh, Aarthi Chandrasekaran, Claudia Pappas, Rahul Raman, Aravind Srinivasan, Zachary Shriver, Terrence M. Tumpey, and Ram Sasisekharan. 2010. Determinants of glycan receptor specificity of H2N2 influenza A virus hemagglutinin. PLoS ONE 5, no. 10: e13768.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4745740
dc.description.abstractThe H2N2 subtype of influenza A virus was responsible for the Asian pandemic of 1957-58. However, unlike other subtypes that have caused pandemics such as H1N1 and H3N2, which continue to circulate among humans, H2N2 stopped circulating in the human population in 1968. Strains of H2 subtype still continue to circulate in birds and occasionally pigs and could be reintroduced into the human population through antigenic drift or shift. Such an event is a potential global health concern because of the waning population immunity to H2 hemagglutinin (HA). The first step in such a cross-species transmission and human adaptation of influenza A virus is the ability for its surface glycoprotein HA to bind to glycan receptors expressed in the human upper respiratory epithelia. Recent structural and biochemical studies have focused on understanding the glycan receptor binding specificity of the 1957-58 pandemic H2N2 HA. However, there has been considerable HA sequence divergence in the recent avian-adapted H2 strains from the pandemic H2N2 strain. Using a combination of structural modeling, quantitative glycan binding and human respiratory tissue binding methods, we systematically identify mutations in the HA from a recent avian-adapted H2N2 strain (A/Chicken/PA/2004) that make its quantitative glycan receptor binding affinity (defined using an apparent binding constant) comparable to that of a prototypic pandemic H2N2 (A/Albany/6/58) HA.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi://10.1371/journal.pone.0013768en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2966429/pdf/en_US
dash.licenseLAA
dc.subjectcell biologyen_US
dc.subjectinfectious diseasesen_US
dc.subjectvirologyen_US
dc.subjecthost invasion and cell entryen_US
dc.subjectvirulence factors and mechanismsen_US
dc.subjectviral infectionsen_US
dc.subjectextra-cellular matrixen_US
dc.titleDeterminants of Glycan Receptor Specificity of H2N2 Influenza A Virus Hemagglutininen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorSasisekharan, Ram
dc.date.available2011-03-19T07:51:40Z
dash.affiliation.otherHMS^Health Sciences and Technologyen_US
dc.identifier.doi10.1371/journal.pone.0013768*
dash.contributor.affiliatedSasisekharan, Ram


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